Expression and function of the ultraspiracle (usp) gene locus during development in Drosophila melanogaster

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Vincent C. Henrich, Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: The usp locus encodes a member of the nuclear hormone receptor superfamily in Drosophila melaaogaater that interacts with EcR (ecdysone receptor) to mediate ecdysteroid-induced gene expression. A 2.7-kb usp mRNA was detected at all developmental times tested, although its abundance varied. Among premetatnorphic stages, both the 2.7-kb transcript and Usp protein attained their highest levels in the late third larval instar. The 2.7-kb usp transcript was also found in adult stages and a 1.2-kb transcript was detected in the polyadenylated RNA fraction of both mature adult females and early embryos. Aneuploids carrying two asp mutant alleles and a putative variegating usp+ allele often developed deformities of the adult wing disc that apparently resulted from mutational disruption of uap activity before metamorphosis and whose frequency was affected by maternal genotype. Both of the recessive lethal uap mutations associated with this "cleft thorax" phenotype involved substitutions of conserved arginine residues in the DNA-binding domain, although the frequency of the phenotype was not the same for the two alleles. Both mutant proteins retained the ability to form heterodimers with EcR in vitro but showed reduced affinity for an ecdysone response element.

Additional Information

Developmental Biology, 165: 38-52
Language: English
Date: 1994
Drosophila melanogaster, Ultraspiracle, Ecdysone receptor

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