|Analysis of transcriptional activity mediated by Drosophila melanogaster ecdysone receptor isoforms in a heterologous cell culture system
||Ecdysteroid regulation of gene transcription in Drosophila melanogaster and other insects is mediated by a heterodimer comprised of Ultraspiracle (USP) and one of three ecdysone receptor (EcR) isoforms (A, B1 and B2). This study revealed that ...
|Arthropod nuclear receptors and their role in molting
||The molting process in arthropods is regulated by steroid hormones acting via nuclear receptor proteins. The most common molting hormone is the ecdysteroid, 20-hydroxyecdysone. The receptors of 20-hydroxyecdysone have also been identified in many art...
|Cell-autonomous roles of the ecdysoneless gene in Drosophila development and oogenesis
||Steroid signaling underlies developmental processes in animals. Mutations that impair steroidogenesis in the fruit fly Drosophila melanogaster provide tools to dissect steroid hormone action genetically. The widely used temperature-sensitive m...
|Characterization of the ligand-binding domain of the ecdysteroid receptor from Drosophila melanogaster
||Mutants created by site-directed mutagenesis were used to elucidate the function of amino acids involved in ligand binding to ecdysteroid receptor (EcR) and heterodimer formation with ultraspiracle (USP). The results demonstrate the importance of the...
|Collagen Gene Variants Previously Associated With Anterior Cruciate Ligament Injury Risk Are Also Associated With Joint Laxity
||Background: Genetic association studies demonstrate a relationship between several collagen gene variants and anterior cruciate ligament (ACL) injury, yet the mechanism of these relationships is still unclear. Joint laxity is a heritable trait; incre...
|Collection of Family Health History for Assessment of Chronic Disease Risk in Primary Care
||Background: Family health history can predict a patient’s risk for common complex diseases. This project assessed the completeness of family health history data in medical charts and evaluated the utility of these data for performing risk assessments...
|A Community’s Awareness and Perceptions of Genomic Medicine
||Background: Focus groups were conducted in a mid-sized community to explore community members’ awareness and perceptions of genomic medicine and identify effective methods to educate the public about this topic. Methods: Thirteen focus groups were co...
|Comparison of Ecdysteroid Production in Drosophila and Manduca: Pharmacology and Cross-Species Neural Reactivity
||In both Manduca sexta and Drosophila melanogaster, metamorphic events are driven by ecdysteroids whose production in prothoracic gland (PGs) is stimulated periodically by neural factors. Differences in the life cycle of moths and flies ...
|Deploying Homeland Security Technology
||Question: What do the Department of Homeland Security, Intel, and Disney have in common?1 Answer: The success of each depends on how efficiently they implement new technologies. Although the White House and the Congress have responded swif...
|Developing SSU rDNA metagenomic profiles of aquatic microbial communities for environmental assessments.
||Five water samples from three sources, two municipal reservoirs in central North Carolina and Toolik Lake in Alaska, were processed to conduct a comparative survey of microbial small subunit rDNA sequences. Genomic DNA was extracted and amplified by ...
|Development and Validation of a Primary Care-Based Family Health History and Decision Support Program (MeTree)
||Introduction: Family health history is a strong predictor of disease risk. To reduce the morbidity and mortality of many chronic diseases, risk-stratified evidence-based guidelines strongly encourage the collection and synthesis of family health hist...
|Developmental Effects of a Chimeric ultraspiracle Gene Derived From Drosophila and Chironomus
||The ultraspiracle (usp) gene encodes a nuclear receptor that forms a heterodimer with the ecdysone receptor (EcR) to mediate transcriptional responses to the insect steroid hormone, 20-hydroxyecdysone (20HE). The responses ultimately el...
|Differential control of gene activity by isoforms A, 131 and 132 of the Drosophila ecdysone receptor
||The steroid hormone ecdysone initiates molting and metamorphosis in Drosophila via a heterodimeric receptor consisting of EcR that binds hormone, and USP, a homolog of the vertebrate RXR receptor. EcR exists in three isoforms EcRA, EcRB1 and EcRB2 th...
|The Ecdysone Receptor Puzzle
||The present article reviews some recent findings on the functional ecdysone† receptor which is a heterodimer of two proteins: ecdysone receptor (EcR) and Ultraspiracle (USP). Emphasis is given to some unique aspects of this receptor, in particular to...
|The Ecdysoneless (ecd1ts) Mutation Disrupts Ecdysteroid Synthesis Autonomously in the Ring Gland of Drosophila melanogaster
||Ring glands dissected from homozygous l(3)ecd1ts wandering larvae and upshifted in vitro to the restrictive temperature, 29°C, synthesize abnormally low quantities of ecdysteroid. Nevertheless, ecd1 ring glands retain the ability to respond at 29°C t...
|Educational Needs of Primary Care Physicians Regarding Direct-to-Consumer Genetic Testing
||To assess the educational needs of North Carolina primary care physicians (PCPs) about direct-to-consumer (DTC) genetic testing, surveys were mailed to 2,402 family and internal medicine providers in North Carolina. Out of 382 respondents, 323 (85%) ...
|Effects of photoperiod on the timing of larval wandering of Drosophila melanogaster
||A rearing method is described that will allow the use of well-timed larvae of Drosophila melanogaster (Canton S) in physiological investigations. Using a LD 16:8 h cycle and uncrowded rearing conditions, it was shown that photoperiod influences the d...
|Expression and function of the ultraspiracle (usp) gene locus during development in Drosophila melanogaster
||The usp locus encodes a member of the nuclear hormone receptor superfamily in Drosophila melaaogaater that interacts with EcR (ecdysone receptor) to mediate ecdysteroid-induced gene expression. A 2.7-kb usp mRNA was detected at all developmental time...
|Feasibility of a bilateral 4000–6000 Hz notch as a phenotype for genetic association analysis
||Objective: Noise-induced hearing loss (NIHL) is a worldwide health problem and a growing concern among young people. Although some people appear to be more susceptible to NIHL, genetic association studies lack a specific phenotype. We tested the feas...
|Findings from a community education needs assessment to facilitate the integration of genomic medicine into primary care.
||Purpose: To assess the lay public's knowledge of, and beliefs about, genetics and genetic testing to create an educational initiative that promotes acceptance and utilization of genomic medicine in primary health care.
Methods: A telephone survey ...
|Functional characterization of two Ultraspiracle forms (CtUSP-1 and CtUSP-2) from Chironomus tentans
||Two forms, CtUSP-1 and CtUSP-2, of the Chironomus tentans homolog of Ultraspiracle (new nomenclature: Chironomus NR2B4) were described and verified as components of the functional ecdysteroid receptor. The two forms differed from each other in the mo...
|Functional studies on the ligand-binding domain of Ultraspiracle from Drosophila melanogaster
||The functional insect ecdysteroid receptor is comprised of the ecdysone receptor (EcR) and Ultraspiracle (USP). The ligand-binding domain (LBD) of USP was fused to the GAL4 DNA-binding domain (GAL4-DBD) and char-acterized by analyzing the effect of s...
|Genetic differences in pupal diapause incidence between two selected strains of the flesh fly
||This study assesses the importance of genetic differences that underlie differences in pupal diapause incidence among two lines of the flesh fly, Sarcophaga bullata. Lines of high and low diapause Incidence were derived through selection and subseque...
|The Genomic Medicine Model: An Integrated Approach to Implementation of Family Health History in Primary Care
||As an essential tool for risk stratification, family health history (FHH) is a central component of personalized medicine; yet, despite its widespread acceptance among professional societies and its established place in the medical interview, its wid...
|Implementing Family Health History Risk Stratification in Primary Care: Impact of Guideline Criteria on Populations and Resource Demand
||The Genomic Medicine Model aims to facilitate patient engagement, patient/provider education of genomics/personalized medicine, and uptake of risk-stratified evidence-based prevention guidelines using MeTree, a patient-facing family health history (F...
|Induction of diapause in Drosophila melanogaster: Photoperiodic regulation and the impact of arrhythmic clock mutations on time measurement
||The fruit fly Drosophila melanogaster displays an ovarian diapause that is regulated by photoperiod. Newly eclosed female flies (Canton-S wild type) exposed to short days (<14 hr of light per day) at 12°C (or 10°C) enter a fairly shallow reproductive...
|Innovative Research Exploring the Effects of Physical Activity and Genetics on Cognitive Performance in Community-Based Older Adults
||Physical activity is predictive of better cognitive performance and lower risk of Alzheimer’s disease (AD). The apolipoprotein E gene (APOE) is a susceptibility gene for AD with the e4 allele being associated with a greater risk of AD. Cross-sectiona...
|Juvenile hormone bisepoxide biosynthesis in vitro by the ring gland of Drosophila melanogaster: A putative juvenile hormone in the higher Diptera
||The in vitro production of juvenile hormone (JH) was investigated by using isolated ring glands from third instar Drosophila melanogaster. A JH-like molecule is secreted that comigrates with a synthetic sample of methyl 6,7;10,11- bisepoxy-3,7,11-tri...
|Juvenile hormone potentiates ecdysone receptor-dependent transcription in a mammalian cell culture system
||Insect development is guided by the combined actions of ecdysteroids and juvenile hormones (JHs). The transcriptional effects of ecdysteroids are mediated by a protein complex consisting of the ecdysone receptor (EcR) and its heterodimeric partner, U...
|Ligand Binding Pocket Function of Drosophila USP is Necessary for Metamorphosis
||The widely accepted paradigm that epoxidized methyl farnesoates (“juvenile hormones,” JHs) are the principal sesquiterpenoid hormones regulating insect metamorphosis was assessed in Drosophila melanogaster. GC–MS analysis of circulating methyl farnes...
|Ligand control of in vivo interaction between ecdysteroid receptor and Ultraspiracle ligand binding domain
||Ecdysteroids (Ecs) enhance the formation of Ec receptor-ultraspiracle protein (EcR-USP) heterodimers which regulate gene transcription. To study EcR-USP heterodimerization, fusion proteins were constructed from the LBDs (ligand-binding domains) of Dr...
|Ligand-induced heterodimerization between the ligand binding domains of the Drosophila ecdysteroid receptor and ultraspiracle
||The insect ecdysteroid receptor consists of a heterodimer between EcR and the RXR-orthologue, USP. We addressed the question of whether this heterodimer, like all other RXR heterodimers, may be formed in the absence of ligand and whether ligand promo...
|A maternal effect that eliminates pupal diapause in progeny of the flesh fly, Sarcophaga bullata
||Flesh flies that have experienced pupal diapause produce progeny that will not enter diapause even when reared in a strongly diapause-inducing environment. The effect is determined, not by diapause itself, but by the short days previously received by...
|The N-terminus of ecdysteroid receptor isoforms and ultraspiracle interacts with different ecdysteroid response elements in a sequence specific manner to modulate transcriptional activity
||The functional insect ecdysteroid receptor is comprised of two nuclear receptors, the ecdysteroid receptor (EcR) and the RXR homologue, ultraspiracle (USP), which form a heterodimer. The dimer recognizes various hormone response elements and the effe...
|Neural factors that stimulate ecdysteroid synthesis by the larval ring gland of Drosophila melanogaster
||The larval ring gland of Drosophila melanogaster is the source of ecdysteroids responsible for larval-larval and larval-pupal molting. An extract prepared from the Drosophila larval central nervous system, that presumably contains prothoracicotropic ...
|A Novel Ecdysone Receptor Mediates Steroid-Regulated Developmental Events during the Mid-Third Instar of Drosophila
||The larval salivary gland of Drosophila melanogaster synthesizes and secretes glue glycoproteins that cement developing animals to a solid surface during metamorphosis. The steroid hormone 20-hydroxyecdysone (20E) is an essential signaling molecule t...
|The Office of Innovation Commercialization and the Promotion & Tenure Process
||In their creative and scholarly work, faculty members occasionally develop ideas for innovations that might lead to the creation of new commercial products or services. In such cases, the faculty
member should consider how patents, trademarks, and c...
|Patient and Primary Care Provider Experience Using a Family Health History Collection, Risk Stratification, and Clinical Decision Support Tool: a Type 2 Hybrid Controlled Implementation-effectiveness Trial
||Background: Family health history (FHH) is the single strongest predictor of disease risk and yet is significantly underutilized in primary care. We developed a patient facing FHH collection tool, MeTree©, that uses risk stratification to generate cl...
|Primary Care Physicians’ Awareness, Experience and Opinions of Direct-to-Consumer Genetic Testing
||The purpose of this study was to assess primary care physicians’ awareness, experience, opinions and preparedness to answer patients’ questions regarding direct-to-consumer (DTC) genetic testing. An anonymous survey was mailed to 2,402 family and int...
|Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates
||Insect development is driven by the action of ecdysteroids on morphogenetic processes. The classic ecdysteroid receptor is a protein heterodimer composed of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspiracle (USP), the insect orthol...
|Protocol for implementation of family health history collection and decision support into primary care using a computerized family health history system
||Background: The CDC's Family History Public Health Initiative encourages adoption and increase awareness of family health history. To meet these goals and develop a personalized medicine implementation science research agenda, the Genomedical Connect...
|Providing Patient Education: Impact on Quantity and Quality of Family Health History Collection
||Background: Family health history (FHH) is an underutilized tool in primary care to identify and risk-stratify individuals with increased cancer risk. Objective: Evaluate the influence of patient education on quantity and quality of FHH entered into ...
|Quality of Family History Collection with Use of a Patient Facing Family History Assessment Tool
||Background: Studies have shown that the quality of family health history (FHH) collection in primary care is inadequate to assess disease risk. To use FHH for risk assessment, collected data must have adequate detail. To address this issue, we develo...
|Selection for late pupariation affects diapause incidence and duration in the flesh fly, Sarcophaga bullata
||This artificial selection study with the flesh fly, Sarcophaga bullata Parker, tested the hypothesis that phenotypic variability in the length of the larval stage (under non-diapause conditions) is largely a consequence of genetic variability. Select...
|A steroid/thyroid hormone receptor superfamily in Drosophila melanogaster that shares extensive sequence similarity with a mammalian counterpart
||A gene in Drosophila melanogaster that maps cytologlcally to 2C1 — 3 on the distal portion of the X-chromosome encodes a member of the steroid/thyrold hormone receptor superfamily. The gene was isolated from an embryonic cDNA library using an oligonu...
|Taxonomy of Pfiesteria (Dinophyceae)
||The dinoflagellate species originally described as Pfiesteria shumwayae Glasgow et Burkholder, recently transferred to a new genus, Pseudopfiesteria Litaker et al., is reclassified into the redefined genus Pfiesteria Steidinger et Burkholder, as Pfie...
|Ultraspiracle promotes the nuclear localization of ecdysteroid receptor in mammalian cells
||The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of...
|An ultrastructural analysis of the ecdysoneless (1(3)ecd1ts) ring gland during the third larval instar of Drosophila melanogaster
||In the late third larval instar of Drosophila melanogaster, the prothoracic gland, an endocrine portion of the ring gland, synthesizes ecdysteroids at an accelerated rate. The resultant ecdysteroid titer peak initiates the events associated with meta...
|The Use of a Family History Risk Assessment Tool within a Community Health Care System: Views of Primary Care Providers
||Primary care providers (PCPs) offered input regarding the incorporation of a family health history (FHH) risk assessment tool into a community health care system (CHCS). Sixteen PCPs participated in one of three focus groups. Perceived impediments in...