|Accurate characterization of carcinogenic DNA adducts using MALDI tandem time-of-flight mass spectrometry.
||Many chemical carcinogens and their in vivo activated metabolites react readily with genomic DNA, and form covalently bound carcinogen-DNA adducts. Clinically, carcinogen-DNA adducts have been linked to various cancer diseases. Among the current meth...
| Base-specific fragmentation of amplified 16S rRNA genes and mass spectrometry analysis: A novel tool for rapid bacterial identification.
||A rapid approach to the 16S rRNA gene (16S rDNA)-based bacterial identification has been developed that combines uracil-DNA-glycosylase (UDG)-mediated base-specific fragmentation of PCR products with matrix-assisted laser desorption ionization-time-o...
|Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein.
||For the development of specific immunological assays, the binding of a specific antibody (Ab) to the target antigen (Ag) has to be relatively strong. In this study, we have utilized affinity capillary electrophoresis (ACE), a form of capillary zone e...
|Comparison of Accuracy on DNA Quantitation Determined by MALDI-TOF Mass Spectrometry and UV Spectrometry
||Although the UV absorbance of DNA at 260 nm has been recognized as a standard method for DNA quantitation, there are limitations of using UV spectrometry to determine the purity and identity of DNA. Recently, MALDI-TOF MS has proven to be an accurate...
|Comparison of Fluorometric Detection Methods for Quantitative Polymerase Chain Reaction (PCR)
||In this study, we compared the sensitivity of two different detection methods for quantitative polymerase chain reaction (PCR). Various amounts of a 75 mer single-stranded deoxyribonucleic acid (DNA) fragment, which can be used as a DNA label for the...
|Expression Immunoassay. Antigen quantitation using antibodies labeled with enzyme-coding DNA fragment.
||A novel immunoassay is reported which uses an enzymecoding DNA fragment as label (expression immunoassay). The DNA label is determined with high sensitivity by measuring the enzymatic activity produced after expression. A DNA fragment encoding the fi...
|The Footprints of Gut Microbial–mammalian Co-Metabolism.
||Gut microbiota are associated with essential various biological functions in humans through a “network” of microbial–host co-metabolism to process nutrients and drugs and modulate the activities of multiple pathways in organ systems that are linked t...
|Heterobifunctional linker between antibodies and reporter genes for immunoassay development
||The amplification inherent in transcription and translation of DNA has already been exploited for the development of highly sensitive immunoassays by using a reporter gene as a label that, upon in vitro expression, generates multiple enzyme molecules...
|Hybridization assays using as a label an expressible DNA fragment encoding firefly luciferase.
||We report the use of a new label, an expressible enzymecoding
DNA fragment, for nucleic acid hybridization
assays. The DNA label contains a firefly luciferase coding
sequence downstream from a T7 RNA polymerase promoter.
The target DNA (200 bp) i...
|Investigation of Enzymatic Behavior of Benzonase/Alkaline Phosphatase in the Digestion of Oligonucleotides and DNA by ESI-LC/MS
||We have developed an ion-pairing HPLC-MS method that has sufficient separation power, selectivity, and sensitivity to investigate the enzymatic behavior of benzonase/alkaline phosphatase upon digestion of oligonucleotides and DNA. Mass spectrometry r...
|Mass spectrometry of single-stranded restriction fragments captured by an undigested complementary sequence.
||In this report, we describe a simple and accurate method to analyze restriction fragments using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The two complementary strands of restriction fragments are separated through...
|Reduction of Internal Standard Signals in Quantitative MALDI-TOF Mass Spectrometry
||The advantages of combining qualitative and quantitative analysis on a single analytical technique have further extended the applications of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to the quantitati...
|A simple approach for evaluating total MicroRNA extraction from mouse brain tissues
||For the analysis of microRNA, a common approach is to first extract microRNA from cellular samples prior to any specific microRNA detection. Thus, it is important to determine the quality and yield of extracted microRNA. In this study, solid-phase ex...
|Specific recognition of non-denatured nitrite-oxidizing system ofNitrospira moscoviensis by monoclonal antibody Hyb 153-3
||The objective of this research project is to develop a rapid molecular method for monitoring nitrification in a wastewater reactor. In the developed method, a monoclonal antibody (Hyb 153-3) was used because it can specifically recognize non-denature...
|Sugar additives for MALDI matrices improve signal allowing the smallest nucleotide change (A:T) in a DNA sequence to be resolved.
||Sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) of DNA is critical for obtaining high quality mass spectra. Sample impurity, solvent content, substrate surface and environmental conditions (temperatur...