Comparison of Accuracy on DNA Quantitation Determined by MALDI-TOF Mass Spectrometry and UV Spectrometry
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Norman H. Chiu, Assistant Professor (Creator)
- Alison Lisa Boyko (Contributor)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
Abstract: Although the UV absorbance of DNA at 260 nm has been recognized as a standard method for DNA quantitation, there are limitations of using UV spectrometry to determine the purity and identity of DNA. Recently, MALDI-TOF MS has proven to be an accurate technique for qualitative DNA analysis. In this study, the accuracy of MALDI-TOF MS for determining the concentration of DNA is evaluated and compared with that of the standard UV method. The results indicated that the accuracy of quantitative MALDI-TOF MS was comparable to that of the standard UV method and that measured DNA concentrations correlated well with those determined by the standard UV method.
Comparison of Accuracy on DNA Quantitation Determined by MALDI-TOF Mass Spectrometry and UV Spectrometry
PDF (Portable Document Format)
147 KB
Created on 12/11/2012
Views: 3897
Additional Information
- Publication
- Language: English
- Date: 2010
- Keywords
- chemistry, biochemistry, MALDI-TOF MS, DNA analysis, mass spectrometry, UV spectrometry