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Mass spectrometry of single-stranded restriction fragments captured by an undigested complementary sequence.

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Norman H. Chiu, Assistant Professor (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: In this report, we describe a simple and accurate method to analyze restriction fragments using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The two complementary strands of restriction fragments are separated through hybridization to a capture probe, which is a single-stranded undigested fragment. Using the biotin–streptavidin linkage, the hybrid is immobilized on streptavidin-coated magnetic beads. After conditioning the captured restriction fragments, they are eluted from the probe and their molecular weights are determined. The proposed method greatly improves the quality, and reduces the complexity of the mass spectrum by analyzing only one of the complementary strands of restriction fragments. Made available courtesy of the Journal of Nucleic Acids Research and Oxford University Press. The version of record is available at: http://nar.oxfordjournals.org/content/28/8/e31.abstract?sid=37143ad3-29aa-440e-9922-2a6989ae20f7

Additional Information

Publication
Language: English
Date: 2000
Keywords
chemistry, biochemistry, mass spectronomy, spectronomy, restriction fragments, nucleic acid