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Regulation of dickkopf-1(Dkk-1) promoter sequences under various growth conditions

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Jasmin Denee Feimster (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Karen Katula

Abstract: "The goal of this study was to determine what region of the Dkk-1 promoter was required for expression of the Dkk-1 gene under different growth conditions. Dkk-1 promoter activity was analyzed in NIH3T3 cell lines stably transfected with luciferase vectors containing 1068, 535, and 228 bp of Dkk-1 upstream sequence that were growing, confluent, serum starved and during the cell cycle. Dkk-1 promoter activity was also analyzed in the cell lines treated with the inhibitors aphidicolin, methotrexate, and U0126. In general, we found that Dkk-1 promoter activity is higher in a growing cell population than in a nondividing population. The 1068 Dkk-1 promoter construct does not appear to change dramatically during the cell cycle nor is it sensitive to an S phase inhibitor or U0126. Methotrexate treatment was found to decrease promoter activity. A sequence region between 1068 and 535 appears to be important for regulation of Dkk-1 promoter activity."--Abstract from author supplied metadata.

Additional Information

Publication
Thesis
Language: English
Date: 2006
Keywords
Dkk-1 promoter, Dkk-1 gene, growth conditions, cell, luciferase vectors
Subjects
Gene expression