The role of increased reactive oxygen species on 3T3-L1 preadipocytes differentiation

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Amit P. Patel (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Yashomati Patel

Abstract: Differentiation of preadipocytes plays an important role in human physiology by accommodating adipocyte expansion and maintaining energy homeostasis. Any process that interferes with preadipocytes differentiation could alter energy homeostasis. In our study, we observed the effect of increased levels of ROS, which has been shown to affect adipocyte functioning, on preadipocytes differentiation. Our study shows that increased levels of ROS can inhibit 3T3-L1 preadipocytes differentiation. While the preadipocytes eventually became resistant to the increased levels of ROS, its effect on differentiation was already fixed. The mechanism by which increased levels of ROS impaired differentiation was by decreasing and delaying Cyclin D1 expression. This would affect the phosphorylation status of retinoblastoma protein which is required for cell cycle progression into the S-phase. Since clonal expansion was halted, the activity and expression of essential transcription factors needed for differentiation C/EBPβ and PPARγ were affected, leading to impaired differentiation. Furthermore, we have shown that once the increased ROS levels are restored to lower amounts, the 3T3-L1 preadipocytes were able to differentiate into mature adipocytes when exposed to MDI. The amount of differentiation observed was diminished compared to the normally differentiating 3T3-L1 preadipocytes, but was significantly greater than cells that kept differentiating in the presence of increased ROS. Overall, we have outlined the timeframe during which oxidative stress impairs 3T3-L1 preadipocyte differentiation. We have shown that, once that increased ROS levels are lowered, differentiation can be restored, but not to the same degree as unstressed differentiating 3T3-L1 preadipocytes.

Additional Information

Language: English
Date: 2012
3T3-L1 preadipocytes, Differentiation, PQ, ROS
Active oxygen $x Physiological effect
Cell differentiation
Fat cells

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