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Simultaneous Determination of 17 Ginsenosides in Rat Urine by Ultra Performance Liquid Chromatography–Mass Spectrometry with Solid-Phase Extraction

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Wei Jia, Professor and Co-Director of the UNCG Center for Research Excellence in Bioactive Food Components (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided.

Additional Information

Publication
Analytica Chimica Acta, 594, 265-273
Language: English
Date: 2007
Keywords
saponin, ginsenoside, solid-phase extraction, rat urine, ultra performance liquid chromatography–mass spectrometry