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Estradiol Reduces Inflammation in Rats Fed a High-fat Diet.

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Colette N. Miller (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Lynda Brown

Abstract: Previous research has shown that leptin and insulin resistance can occur after rats are fed a high-fat (HF) diet for 72 hours. Because leptin and insulin resistance can be a result of HF diet-induced inflammation, the purpose of this research was to determine if inflammatory gene expression occurs after 72 hours of a HF diet. Additionally, since estrogen (E2) is anti-inflammatory, the extent of which intact females and ovariectomized (OVX) express inflammatory cytokines after 72 hours of a HF diet was also determined.

Intact females in proestrus had reduced hypothalamic inflammatory gene expression of TNFa, whereas males had increased hypothalamic expression of SOCS3 after 72 hours of a HF diet. Within the liver, females in proestrus had reduced expression of all genes measured in addition to reduced XBP1 mRNA after a HF diet. However, no such reductions were observed within males.

To determine if these reductions in inflammatory gene expression was due to the increased circulating E2 seen during proestrus, E2 was reintroduced in an OVX model. After 24 hours of a HF diet, E2 treatment prevented increases in hypothalamic SOCS3 expression. However, this protection was attenuated at 72 hours and no other treatmentinduced changes were observed.

Additional Information

Publication
Thesis
Language: English
Date: 2010
Keywords
estrogen, high-fat diets, inflammation, insulin, leptin, obesity
Subjects
Rats $x Physiology $x Research.
Inflammation.
Estradiol.
Leptin $x Physiology.