Identification of commensal bacteria autoinducing peptides with ultrahigh performance liquid chromatography - high resolution mass spectrometry

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Luis A. Mejia Cruz (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Nadja Cech

Abstract: Staphylococcus aureus is an opportunistic pathogen that has become an increasing issue over the years due to its acquired resistance to different antibiotics such as methicillin. The development of resistance has sparked interest in finding new ways to combat S. aureus, and other pathogens. Recent hypotheses suggest that different types of commensal bacteria in the skin are able to cross-inhibit virulence in S. aureus, by suppressing the production of its virulence factors responsible for disease. Cross-inhibition is believed to involve autoinducing signaling molecules that are unique to different bacterial species. These signaling molecules are responsible for self-regulating functions in a bacterium that produces them and for potentially disrupting similar processes in other species. More knowledge is needed about how these signaling molecules from commensal bacteria can affect quorum sensing in the bacterial pathogen S. aureus, however, in order to further study these interactions, the properties of the signaling molecules must be known, starting with their molecular structure. The goal of this project is to detect and elucidate the structures of unknown signaling molecules from different commensal staphylococcal species that have previously displayed cross-inhibition activity against quorum sensing in S. aureus.

Additional Information

Language: English
Date: 2020
agr, Autoinducing Peptides, Mass Spectrometry, Quorum sensing, Staphylococcus
Staphylococcus aureus
Cyclic peptides
Quorum sensing (Microbiology)

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