Protein Trapping in Plasmonic Nanoslit and Nanoledge Cavities: The Behavior and Sensing

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Taylor Levi Mabe (Creator)
Jianjun Wei, Associate Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: A novel plasmonic nanoledge device was presented to explore the geometry-induced trapping of nanoscale biomolecules and examine a generation of surface plasmon resonance (SPR) for plasmonic sensing. To design an optimal plasmonic device, a semianalytical model was implemented for a quantitative analysis of SPR under plane-wave illumination and a finite-difference time-domain (FDTD) simulation was used to study the optical transmission and refractive index (RI) sensitivity. In addition, total internal reflection fluorescence (TIRF) imaging was used to visualize the migration of fluorescently labeled bovine serum albumin (BSA) into the nanoslits; and fluorescence correlation spectroscopy (FCS) was further used to investigate the diffusion of BSA in the nanoslits. Transmission SPR measurements of free prostate specific antigen (f-PSA), which is similar in size to BSA, were performed to validate the trapping of the molecules via specific binding reactions in the nanoledge cavities. The present study may facilitate further development of single nanomolecule detection and new nanomicrofluidic arrays for effective detection of multiple biomarkers in clinical biofluids. [The original abstract for this article contains (characters/images) that cannot be displayed here. Please click on the link below to read the full abstract and article.]

Additional Information

Analytical Chemistry, 2017, 89 (10), 5221–5229. DOI: 10.1021/acs.analchem.6b04493
Language: English
Date: 2017
surface plasmon resonance (SPR), nanomolecule, finite-difference time-domain (FDTD)

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