The effects of refrigerated storage time and fortification on pasteurized donor human milk

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Hannah Rose Schlotterer (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Maryanne Perrin

Abstract: Background: The use of pasteurized donor human milk (PDHM) is growing among neonatal intensive care units. Addition of commercial fortifiers to better meet the nutritional needs of preterm infants is common, however limited information is available regarding nutrient stability in fortified PDHM (FPDHM). Objective: The purpose of this study is to measure the anti-microbial activity and protein content of FPDHM during refrigerated storage over 96 hours. Methods: Unfortified PDHM served as the control (CONTROL). PDHM was subjected to treatment with 3 different fortifiers: an acidic (F-ACID), a neutral (F-NEUT), and a human-milk derived (F-HUM) fortifier. Samples were stored at 4°C, and every 24h, a 1-mL aliquot was removed for analysis. Samples were analyzed for total protein, immunoglobulin A (IgA), and lysozyme. Results: At baseline, there was a significant difference in protein (mean, standard deviation) concentration (g/dL) between control (1.3, 0.14) and all other treatments (F-ACID = 2.0, 0.19; F-NEUT= 2.2, 0.14 ; F-HUM = 2.5, 0.12; p<0.001). Lysozyme and IgA were significantly lower in the F-ACID group (p<0.001). Lysozyme and IgA were significantly higher in the F-HUM group (p<0.001). There was no significant effect of time (p>0.9 all variables), nor was there a significant interaction effect between time and treatment (p>0.9 all variables). Conclusion: The type of fortifier has a more significant impact on bioactive components in fortified PDHM than storage time.

Additional Information

Publication
Thesis
Language: English
Date: 2019
Keywords
Donor human milk, Donor milk, Fortified, Storage
Subjects
Breast milk $x Collection and preservation
Breast milk $x Composition
Premature infants $x Nutrition

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