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A HPLC/ESI-MS method developed and validated to evaluate the quantity, identity, and stability of the alkylamides in ethanolic extracts of Spilanthes acmella

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Stacy S. Bae (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Nadja B Cech

Abstract: "In order to evaluate the efficacy of herbal drugs, there need to be ways to qualitatively and quantitatively determine the constituents and their concentrations in various products. Spilanthes is a popular herbal extract which is used traditionally to treat stammering, stomatistis, and throat complaints. Spilanthes is commonly referred to as the "toothache plant," a name that refers to the analgesic action of its alkylamide constituents. A high performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) method was developed to evaluate the quantity, identity, and stability of the alkylamides in ethanolic extracts of Spilanthes acmella. The developed method was then subjected to method validation according to guidelines given by the International Committee of Harmonisation (ICH) section Q2 (R1). The linear dynamic range for the method was determined to be 0.45 uM- 450 uM. The method detection limit and quantitation limit were estimated at 0.27 uM and 0.45 uM, respectively. The method precision was evaluated on two levels: repeatability and intermediate precision and was 6% or less. The method proves valuable for the quantitation and identification of the alkylamide spilanthol in S. acmella. Eight additional alkylamides have been tentatively identified (based on molecular weight and fragmentation patterns) in a 75% ethanol extract of S. acmella. The structure of the most abundant of these alkylamides, (2E,6Z,8E)-N-isobutyl-2,6,8-decatrienamide, spilanthol, was confirmed by NMR. The spilanthol concentration in the extract shows no significant degradation during storage over six and a half months at room temperature, -20°C, and -80°C. Spilanthes extract and isolated spilanthol showed no antibacterial activity against Staphylococcus aureus."--Abstract from author supplied metadata.

Additional Information

Publication
Thesis
Language: English
Date: 2007
Keywords
Herbal, drugs, efficacy, constituents, concentrations, products, Spilanthes
Subjects
Spilanthes
Herbs--Therapeutic use