Huzefa A. Raja

  • Postdoctoral Fellow
  • Chemistry and Biochemistry, UNCG

There are 39 included publications by Huzefa A. Raja :

TitleDateViewsBrief Description
a-Pyrone Derivatives, Tetra/Hexahydroxanthones, and Cyclodepsipeptides from Two Freshwater Fungi 2017 11 Eighteen (1–18) and seven (1, 4, 6–8, 17 and 18) compounds were isolated from organic extracts of axenic cultures of two freshwater fungi Clohesyomyces sp. and Clohesyomyces aquaticus (Dothideomycetes, Ascomycota), respectively. Compounds 1–12 belong...
Acetophenone Derivatives from a Freshwater Fungal Isolate of Recently Described Lindgomyces Madisonensis (G416) 2016 14 The exploration of freshwater ascomycetes, which have undergone only limited investigation, may provide opportunities both to characterize new genera/species of fungi and to uncover new chemical diversity. In this study, seven acetophenone derivative...
Additions to Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes), Including Two New Species Occurring on Submerged Wood from North Carolina, USA, with Notes on Secondary Metabolite Profiles 2017 13 Two new species of freshwater ascomycetes belonging to the genus Lindgomyces (Pleosporales, Dothideomycetes) are described and illustrated from submerged wood in North Carolina, USA. Lindgomyces carolinensis is characterized by immersed to erumpent a...
Antimicrobial Fungal Endophytes from the Botanical Medicine Goldenseal (Hydrastis canadensis) 2016 9 The potential of fungal endophytes to alter or contribute to plant chemistry and biology has been the topic of a great deal of recent interest. For plants that are used medicinally, it has been proposed that endophytes might play an important role in...
Benzoquinones and Terphenyl Compounds As Phosphodiesterase-4B Inhibitors from a Fungus of the Order Chaetothyriales (MSX 47445) 2013 618 Three bioactive compounds were isolated from an organic extract of an ascomycete fungus of the order Chaetothyriales (MSX 47445) using bioactivity-directed fractionation as part of a search for anticancer leads from filamentous fungi. Of these, two w...
Biosynthesis of Fluorinated Peptaibols Using a Site-Directed Building Block Incorporation Approach 2017 15 Synthetic biological approaches, such as site-directed biosynthesis, have contributed to the expansion of the chemical space of natural products, making possible the biosynthesis of unnatural metabolites that otherwise would be difficult to access. S...
Biosynthetically Distinct Cytotoxic Polyketides from Setophoma terrestris 2015 13 Sixteen polyketides belonging to diverse structural classes, including monomeric/dimeric tetrahydroxanthones and resorcylic acid lactones, were isolated from an organic extract of a fungal culture Setophoma terrestris (MSX45109) by bioactivity-direct...
Chemoselective fluorination and chemoinformatic analysis of griseofulvin: Natural vs fluorinated fungal metabolites 2017 27 [2017-2018 UNCG University Libraries Open Access Publishing Fund Grant Winner.] Griseofulvin is a fungal metabolite and antifungal drug used for the treatment of dermatophytosis in both humans and animals. Recently, griseofulvin and its analogues hav...
Comparison of the chemistry and diversity of endophytes isolated from wild-harvested and greenhouse-cultivated yerba mansa (Anemopsis californica) 2015 396 With this study, we explored the identity and chemistry of fungal endophytes from the roots of yerba mansa [Anemopsis californica (Nutt.) Hook. & Arn. (Saururaceae)], a botanical traditionally used to treat infection. We compared the diversity of fun...
Dereplicating and Spatial Mapping of Secondary Metabolites from Fungal Cultures in Situ 2015 530 Ambient ionization mass spectrometry techniques have recently become prevalent in natural product research due to their ability to examine secondary metabolites in situ. These techniques retain invaluable spatial and temporal details that are lost th...
DNA Barcoding for Identification of Consumer-Relevant Mushrooms: A Partial Solution for Product Certification? 2017 11 One challenge in the dietary supplement industry is confirmation of species identity for processed raw materials, i.e. those modified by milling, drying, or extraction, which move through a multilevel supply chain before reaching the finished product...
Enhanced Dereplication of Fungal Cultures Via Use of Mass Defect Filtering 2017 8 Effective and rapid dereplication is a hallmark of present-day drug discovery from natural sources. This project strove to both decrease the time and expand the structural diversity associated with dereplication methodologies. A 5?min liquid chromato...
Evaluation of culture media for the production of secondary metabolites in a natural products screening program 2013 1017 Variation in the growing environment can have significant impacts on the quantity and diversity of fungal secondary metabolites. In the industrial setting, optimization of growing conditions can lead to significantly increased production of a compoun...
Flavonolignans from Aspergillus iizukae, a Fungal Endophyte of Milk Thistle (Silybum marianum) 2014 753 Silybin A (1), silybin B (2), and isosilybin A (3), three of the seven flavonolignans that constitute silymarin, an extract of the fruits of milk thistle (Silybum marianum), were detected for the first time from a fungal endophyte, Aspergillus iizuka...
Freshwater Ascomycetes: Minutisphaera (Dothideomycetes) revisited, including one new species from Japan 2013 851 During investigations of freshwater ascomycetes we found one interesting taxon from Aomori (Japan), as well as three additional taxa from North Carolina (USA), which were morphologically similar to Minutisphaera, a recently described freshwater fungu...
Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community 2017 12 Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They are known to produce numerous bioactive molecules, which makes them very useful for natural products researchers in their pursuit of discovering new chemical d...
Fungal Planet Description Sheets: 371-399 2015 5 Novel species of fungi described in the present study include the following from Australia:Neoseptorioides eucalypti gen. & sp. nov. from Eucalyptus radiata leaves, Phytophthoragondwanensis from soil, Diaporthe tulliensis from rotted stem ends of The...
Greensporones: Resorcylic Acid Lactones from an Aquatic Halenospora sp. 2014 565 Fourteen new resorcylic acid lactones (1–14) were isolated from an organic extract of a culture of a freshwater aquatic fungus Halenospora sp. originating from a stream in North Carolina. The structures were elucidated using a set of spectroscopic an...
Isochromenones, isobenzofuranone, and tetrahydronaphthalenes produced by Paraphoma radicina, a fungus isolated from a freshwater habitat 2014 787 Six isochromenones (1–6), clearanols F (5) and G (6), one isobenzofuranone (7), and two tetrahydronaphthalene derivatives (8 and radinaphthalenone (9)), were isolated and identified from a culture of the fungus Paraphoma radicina, which was isolated ...
Lindgomyces angustiascus, (Lindgomycetaceae, Pleosporales, Dothideomycetes), a new lignicolous species from freshwater habitats in the USA 2013 713 In this paper, we describe and illustrate a new species, Lindgomyces angustiascus, from submerged wood in lotic and lentic habitats from Florida, North Carolina and Wisconsin, USA. The new species is characterized by black, partially immersed, flatte...
Mass Spectrometry Imaging of Secondary Metabolites Directly on Fungal Cultures 2014 9 Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient ionization technique that enables imaging experiments directly on fungal cultures. Much information can be gained by examining an organism directly from culture, rather than...
Meroterpenoids from Neosetophoma sp.: A Dioxa[4.3.3]propellane Ring System, Potent Cytotoxicity, and Prolific Expression 2019 10 Six fungal metabolites, of which five were new, including one (1) with a dioxa[4.3.3]propellane ring system, were discovered, identified, and structurally elucidated from Neosetophoma sp. (strain MSX50044); these compounds are similar to the bis-trop...
Minutisphaerales (Dothideomycetes, Ascomycota): a new order of freshwater ascomycetes including a new family, Minutisphaeraceae, and two new species from North Carolina, USA 2015 899 Minutisphaera is a recently established genus of freshwater Dothideomycetes characterized by small, globose to subglobose or apothecioid, erumpent to superficial, brown ascomata; fissitunicate, eight-spored, ovoid to obclavate asci; and 1–2-septate, ...
New Diketopiperazine Dimer from a Filamentous Fungal Isolate of Aspergillus sydowii 2015 8 Prior investigations of filamentous fungi in our group have resulted in the isolation of several new and biologically active natural products.[1-3] Continuing these investigations, we have now analyzed the metabolites from a fungal isolate of Aspergi...
Optimizing Production and Evaluating Biosynthesis in Situ of a Herbicidal Compound, Mevalocidin, from Coniolariella Sp. 2016 14 Mevalocidin is a fungal secondary metabolite produced by Coniolariella sp. It is a unique phytotoxin that demonstrates broad spectrum post-emergent herbicidal properties. With limited options for weed control, the commercialization of a natural produ...
Pannorin B, a New Naphthopyrone from an Endophytic Fungal Isolate of Penicillium Sp. 2016 10 The herbal remedy, milk thistle (Silybum marianum), has been used in traditional medicine for various liver, kidney, and gall bladder ailments. For over a decade, our research group has been investigating the flavonolignans obtained from this medicin...
Peptaibols, Tetramic Acid Derivatives, Isocoumarins, and Sesquiterpenes from a Bionectria sp. (MSX 47401) 2013 560 An extract of the filamentous fungus Bionectria sp. (MSX 47401) showed both promising cytotoxic activity (>90% inhibition of H460 cell growth at 20 µg/mL) and antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). A bioact...
Phylogenetic and chemical diversity of fungal endophytes isolated from Silybum marianum (L) Gaertn. (milk thistle) 2015 658 Use of the herb milk thistle (Silybum marianum) is widespread, and its chemistry has been studied for over 50 years. However, milk thistle endophytes have not been studied previously for their fungal and chemical diversity. We examined the fungal end...
Polyhydroxyanthraquinones as Quorum Sensing Inhibitors from the Guttates of Penicillium restrictum and Their Analysis by Desorption Electrospray Ionization Mass Spectrometry 2014 818 The endophytic fungus Penicillium restrictum was isolated from the stems of a milk thistle (Silybum marianum) plant. In culture, the fungus produced distinct red guttates, which have been virtually uninvestigated, particularly from the standpoint of ...
Prealamethicin F50 and Related Peptaibols from Trichoderma arundinaceum: Validation of their Authenticity via in situ Chemical Analysis 2017 15 In the field of natural products chemistry, a common question pertains to the authenticity of an isolated compound, i.e. are the interesting side chains biosynthesized naturally or an artefact of the isolation/purification processes? The droplet-liqu...
qNMR for Profiling the Production of Fungal Secondary Metabolites 2017 11 Analysis of complex mixtures is a common challenge in natural products research. Quantitative nuclear magnetic resonance spectroscopy offers analysis of complex mixtures at early stages and with benefits that are orthogonal to more common methods of ...
Recommendations for competing sexual-asexually typified generic names in Sordariomycetes (except Diaporthales, Hypocreales, and Magnaporthales) 2016 1 With the advance to one scientific name for each fungal species, the generic names in the class Sordariomycetes typified by sexual and asexual morphs are evaluated based on their type species to determine if they compete with each other for use or pr...
Safety Assessment of Mushrooms in Dietary Supplements by Combining Analytical Data with in Silico Toxicology Evaluation 2017 11 Despite growing popularity in dietary supplements, many medicinal mushrooms have not been evaluated for their safe human consumption using modern techniques. The multifaceted approach described here relies on five key principles to evaluate the safet...
Sorbicillinoid Analogs with Cytotoxic and Selective Anti-Aspergillus Activities from Scytalidium album 2014 6 As part of an ongoing project to explore filamentous fungi for anticancer and antibiotic leads, 11 compounds were isolated and identified from an organic extract of the fungus Scytalidium album (MSX51631) using bioactivity-directed fractionation agai...
Spatial and Temporal Profiling of Griseofulvin Production in Xylaria cubensis Using Mass Spectrometry Mapping 2016 12 A large portion of natural products research revolves around the discovery of new, bioactive chemical entities; however, studies to probe the biological purpose of such secondary metabolites for the host organism are often limited. Mass spectrometry ...
Spiroscytalin, a New Tetramic Acid and Other Metabolites of Mixed Biogenesis from Scytalidium cuboideum 2015 13 Spiroscytalin (1), a new tetramic acid that possesses an uncommon spiro-ring fusion between a polyketide-derived octalin ring system and a 2,4-pyrrolidinedione, along with two known compounds, leporin B (2) and purpactin A (3), were isolated from a s...
Talarolutins A–D: Meroterpenoids from an Endophytic Fungal Isolate of Talaromyces minioluteus 2016 13 Four meroterpenoids [talarolutins A–D] and one known compound [purpurquinone A] were characterized from an endophytic fungal isolate of Talaromyces minioluteus (G413), which was obtained from the leaves of the medicinal plant milk thistle [Silybum ma...
w-Hydroxyemodin Limits Staphylococcus aureus Quorum Sensing-Mediated Pathogenesis and Inflammation 2015 785 Antibiotic-resistant pathogens are a global health threat. Small molecules that inhibit bacterial virulence have been suggested as alternatives or adjuncts to conventional antibiotics, as they may limit pathogenesis and increase bacterial susceptibil...
Waol A, trans-dihydrowaol A, and cis-dihydrowaol A: polyketide-derived (gamma)-lactones from a Volutella species 2013 768 An organic extract of a filamentous fungus (MSX 58801), identified as a Volutella sp. (Hypocreales, Ascomycota), displayed moderate cytotoxic activity against NCI-H460 human large cell lung carcinoma. Bioactivity-directed fractionation led to the iso...