Keratin modulation of autophagic flux in nutrient deprived HEK-293 cells

WCU Author/Contributor (non-WCU co-authors, if there are any, appear on document)
W. Zane Billings (Creator)
Western Carolina University (WCU )
Web Site:
Heather Coan

Abstract: Keratose is an oxidized form of keratin which can be extracted from hair, and is composed of alpha-keratin filaments and smaller gamma-keratins. Previous studies have shown keratin's ability to assist in burn healing and rescue stressed cells. Additionally, gamma-keratin was related to the upregulation of autophagy-associated genes in heat shocked cells, suggesting that the therapeutic value of keratin as a biomaterial may be explained by the induction of autophagy by keratins. Our study of keratin treatment in HEK-293 cells revealed that different protein treatments cause levels of fluorescent puncta (measuring the progress of autophagic flux in transfected cells) to change differently over time. Overall, our research suggests that keratin may be modulating autophagic flux, but slightly different experimental methods and further replication would make the link more clear.

Additional Information

Language: English
Date: 2020
keratin, autophagic flux, HEK-293

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