Comparative thermal properties of aryl-B-Glucosidase isozymes in Neurospora crassa

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Eileen Harley Hartis (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Bruce Eberhart

Abstract: Thin layer gel filtration of crude conidial washes from 26 different strains of Neurospora crassa demonstrated three distinct areas of 8-glucosidic activity. The area farthest from the origin was the largest enzyme, aryl-B-glucosidase "Y". The second, smaller isozyme "W" was located closer to the origin, and the third, smallest, newly discovered isozyme, designated "V", was closest to the origin. The new aryl-B-glucosidase "V" was present in extremely low concentration, and required 9-12 hours incubation with substrate (4-methyl-umbelliferyl-B-D-glucopyranoside) in order to note activity. Attempts to purify and isolate "V" were only partially successful.

Additional Information

Language: English
Date: 1975
Neurospora crassa

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