Label-Free Detection of DNA Hybridization with A Compact LSPR-based Fiber-Optic Sensor

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Jianjun Wei, Associate Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
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Abstract: A miniaturized, robust, localized surface plasmon resonance (LSPR)-coupled fiber-optic (FO) nanoprobe providing an integrated and portable solution for detection of DNA hybridization and measurement of DNA concentrations has been demonstrated. The FO nanoprobe was created by constructing arrays of metallic nanostructures on the end facets of optical fibers utilizing nanofabrication technologies, including electron beam lithography and lift-off processes. The LSPR-FO nanoprobe device offers real-time, label-free, and low-sample-volume quantification of single-strand DNA in water with high sensitivity and selectivity, achieving a limit of detection around 10 fM. These results demonstrate the feasibility of the LSPR-FO nanoprobe device as a compact and low-cost biosensor for detection of short-strand DNA.

Additional Information

The Analyst, 2017, 142, 1974-1981. DOI: 10.1039/C7AN00249A
Language: English
Date: 2017
localized surface plasmon resonance (LSPR), fiber optic (FO), short strand DNA detection, nanoprobes

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