Comparative thermal properties of aryl-B-Glucosidase isozymes in Neurospora crassa
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Eileen Harley Hartis (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Bruce Eberhart
Abstract: Thin layer gel filtration of crude conidial washes from 26 different strains of Neurospora crassa demonstrated three distinct areas of 8-glucosidic activity. The area farthest from the origin was the largest enzyme, aryl-B-glucosidase "Y". The second, smaller isozyme "W" was located closer to the origin, and the third, smallest, newly discovered isozyme, designated "V", was closest to the origin. The new aryl-B-glucosidase "V" was present in extremely low concentration, and required 9-12 hours incubation with substrate (4-methyl-umbelliferyl-B-D-glucopyranoside) in order to note activity. Attempts to purify and isolate "V" were only partially successful.
Comparative thermal properties of aryl-B-Glucosidase isozymes in Neurospora crassa
PDF (Portable Document Format)
5433 KB
Created on 1/1/1975
Views: 181
Additional Information
- Publication
- Thesis
- Language: English
- Date: 1975
- Subjects
- Neurospora crassa
- Isoenzymes