Investigation of the relationship of tip extension and cellulase secretion in Neurospora Crassa

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Kathy Martin Goolsby (Creator)
The University of North Carolina at Greensboro (UNCG )
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Bruce Eberhart

Abstract: The relationship of the extension of hyphal tips to secretion was examined in several strains of Neurospora crassa. Cellulase, an extracellular enzyme, was chosen as an index of protein secretion. The plan of this thesis was to examine the following points related to secretion: 1. Simplification of the assay of cellulase activity by improving existing methods and by originating other methods. 2. Determination of the number of cellulases present in induced mycelial and conidial cultures. 3. Study of factors which influence the rate of tip extension. 4. Determination if tip extension is necessary for secretion. As part of the assay of cellulase activity, improvements were made in the Somogyi reducing sugar method. Several limitations of this method were identified including the inhibitory effect of high protein concentrations and the Inhibitory effect of Vogel's and of Fries media. The Worthington Glucostat reagent, in oowbination with excess B-glucosidase, was used to develop a spot test for cellulase activity and to determine the location of cellulase activity for thin-layer gel separations. An additional variation of the Glucostat method was developed using homovanillic acid rather than the standard Glucostat chromogen for a fluorescent determination of cellulase activity.

Additional Information

Language: English
Date: 1974
Neurospora crassa $x Growth
Cellulase $x Secretion

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