A modifier of [beta]-glucosidases in neurospora crassa
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Carroll Christian Miller (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Bruce Eberhart
Abstract: Studies of a now suppressor effect on the gluc-2 gene are reported here. It is believed this effect is due to the su-1 gene. Genetic studies of su-1 indicated initially single gene action at a locus other than gluc-2. The gluc-2 regulatory gene in turn may have a super-repressor effect, since it repressed aryl-B-glucosidase activity to less than one-percent of wild-type levels. The suppressed gluc-2 mutant appeared to be a revertant to wild-type, gluc-2+. The phenotypic wild-type mutant, strain, 12-2-68FA, was selected by ultraviolet irradiation of conidia from a gluc-2, inos, cot, "A" strain, subsequent screening by a modified "inositol-less death" technique, and recovery of a phenotypic gluc-2+ colony. The genotype of the mutant strain recovered was gluc-2, su-1 , leu, inos, "A". The cot mutation had changed to cot+ . Vegetative reisolation of individual conidia revealed homocaryosis for leu, cot, inos, and gluc-2+ phenotypes. Heterocaryon tests showed gluc-2 to be dominant to its wild-type allele, gluc-2+ . The wild-type allele, cot+, was shown to be dominant to cot; su-1 was recessive to su-1+ in a heterocaryon. Aryl-B-glucosidase levels regulated by the mutant were initially greater than wild-type as shown by qualitative tests and quantitative enzyme assays. Preliminary electrophoretic studies revealed no different pattern from a normal wild-type strain of N. crassa.
A modifier of [beta]-glucosidases in neurospora crassa
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Created on 1/1/1971
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Additional Information
- Publication
- Thesis
- Language: English
- Date: 1971
- Subjects
- Neurospora crassa
- Glucosides