A pilot study of the visible effects of estradiol on human intestinal cells in tissue culture

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Linda Neskaug Curtis (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Laura Anderton

Abstract: The purpose of this tissue culture study was to determine whether physiological levels of the steroid 17-B - estradiol would stimulate visible changes in morphology or growth of adult human fibroblastic cells derived from the large intestine, a non-target tissue. Two methods of data collection were used. First, sequential phase-contrast photomicrographs were made of 2 groups of 9 secondary cultures of 79-8924 tissue in plastic flasks at selected times during 3-day intervals. At the end of each experiment, cell monolayers were fixed with 10% neutral buffered formalin. Flasks were rinsed, sawed into 1 X 2½ inch slides and, stained with a modification of Lehman's (1965) polychrome staining procedure to which an initial staining period in 1% Alcian Blue and the final treatment with oil of cedar for clearing and mounting were added for the second type of data collection. This consisted of bright-field microscopic observation of stained slides. Analysis of stained slides included determination of mitotic coefficients, enumeration of lavender- and yellow-staining nuclei among the expected steel blue nuclei, and calculation of nuclear size index values as the product of the greatest length X greatest width of 90 oval nuclei per slide. The t-test was used to compare the difference of pairs of means.

Additional Information

Language: English
Date: 1971
Human cell culture

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