Effects of dietary and physiological factors on Fe transport and metabolism by human intestinal Caco-2 cells

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Okhee Han (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Mark L. Failla

Abstract: The Caco-2 human intestinal cell line was selected as a potential model for the study of iron bioavailability and absorption because confluent cultures spontaneously differentiate into polarized, enterocyte-like cells. Initially, the influence of several dietary factors on Fe uptake and transport by Caco-2 intestinal cells were examined. Both the uptake and transport of nonheme Fe were decreased by inositol hexaphosphate and its lesser phosphorylated metabolites. In contrast, ascorbic acid enhanced the cellular acquisition and transport of this micronutrient, even in the presence of inositol phosphates. These and other data support the usefulness of the Caco-2 cell line as an appropriate model for investigating qualitative and quantitative effects of various dietary factors on iron bioavailability for humans.

Additional Information

Publication
Dissertation
Language: English
Date: 1995
Subjects
Intestinal absorption
Iron $x Metabolism
Iron $x Physiological transport

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