Cytotoxicity of engineered nanoparticles used in industrial processing

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Steven Crawford (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
James Ryan

Abstract: Engineered nanoparticles (NPs) are now heavily used in industrial processing where they are eliminated as waste after use. This waste is a mix of used nanoparticles and process byproducts. While research continues to be done on the toxicity of NPs due to size and composition of pristine material, waste NPs from industrial processes are likely to have modified properties that impact their level of toxicity. These studies investigate this transformation in physicochemical properties that has not been adequately explored by examining waste from relevant high-volume chemical mechanical planarization (CMP) processes used by the semiconductor industry. New (pristine) polish slurries and generated waste samples from various key CMP processes are fully characterized for relevant physicochemical properties to determine any transformation of NPs due to processing. Additionally, high throughput in vitro microplate-based assays assess the toxicity, oxidative stress, and mode of cell death for nanoparticles in both pristine and waste slurries to highlight any differences in biological effects. A combination of darkfield microscopy and inductively coupled plasma optical emission spectroscopy (ICP-OES) indicate cellular uptake of slurry nanoparticles. The results of this study explore the type, magnitude, and biological effect of transformed nanoparticles in CMP waste. The results presented support nanoparticle transformation as an important facet to consider in the risk assessment for new materials.

Additional Information

Language: English
Date: 2019
Chemical Mechanical Planarization, Cytotoxicity, Nanoparticle, Physicochemical characterization, Slurry, Wastewater
Nanoparticles $x Toxicology
Chemical mechanical planarization
Manufacturing processes

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