In vivo and in vitro effects of retinoids on the histological changes in colorectal tissue

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Patricia Bowling Brevard (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site:
Aden C. Magee

Abstract: The purpose of this study was to determine the histological changes induced by high physiological levels of dietary retinoids on rat colorectal tissue in vivo and on human colorectal adenomas and adenocarcinomas in vitro. For the in vivo study, young male rats were fed stock diets supplemented with 6, 30, or 60 Retinol Equivalents (RE) of retinol (Retinol A, B, and C, respectively) or $-carotene (0-carotene A, B, and C, respectively) for two weeks. The animals were sacrificed arid a segment of colorectal tissue was fixed at the end of the experiment. The High Iron Diamine-Alcian Blue and Periodic Acid Thionin/Potassium Hydroxide/Periodic Acid Schiff stains were used to determine the numbers of goblet cells and mast cells and amount, type, and location of mucus produced by goblet cells and connective tissue. The in vitro study was performed on human tumors exposed to retinol (75 or 250 ug/100 ml medium, Retinol 1 and 2, respectively) and B-carotene (250 or 500 ug/100 ml medium, B-carotene 1 and 2, respectively), for six days. Histological stains and dependent variables were the same as; the in vivo study. Number of plasma cells and lymphocytes were also determined.

Additional Information

Language: English
Date: 1984
Retinoids $x Experiments
Colon (Anatomy)

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