Identifying oviposition attractants from the larval rearing medium of phlebotomus papatasi, the vector of old world zoonotic cutaneous leishmaniasis
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Bahjat Fadi Marayati (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Gideon Wasserberg
Abstract: Phlebotomine sand flies are the vectors of the Leishmania parasites as well as other bacterial and viral pathogens worldwide. Due to the variable impact of traditional vector control practices, a more ecologically-based approach is needed. The goal of this study is to develop an attractive blend that would attract gravid Phlebotomus papatasi female sand flies and that would be later used as a lure for a lethal oviposition trap. The general hypothesis of this study is that female sand flies have evolved to detect cues of bacterial origin indicating suitable growth habitat for its progeny. Since sand fly larvae feed on decomposing organic material of predominantly fecal origin, 5 candidate materials were tested; rabbit feces, larval food, 2nd/3rd instar rearing medium, 4th/pupae rearing medium, and expired colony medium. The total oviposition response of each of the candidates was tested using a multiple-choice bioassay, and their relative attractance was tested using a 2-choice attraction bioassay. The 2nd/3rd instar rearing medium demonstrated the highest significant effect in all bioassays and, therefore, a bacterial analysis was performed in order to culture all constituent bacterial isolates. 2-choice attraction bioassays were used to test the attractance of each of the bacterial isolates at serially-diluted concentrations. Certain bacterial isolates have demonstrated high attractance at specific concentrations, which indicates potential applications in constructing the optimal attractive bait.
Identifying oviposition attractants from the larval rearing medium of phlebotomus papatasi, the vector of old world zoonotic cutaneous leishmaniasis
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Created on 8/1/2015
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Additional Information
- Publication
- Thesis
- Language: English
- Date: 2015
- Keywords
- Attraction, Bioassay, Leishmaniasis, Oviposition, Phlebotomus papatasi, Sand fly
- Subjects
- Phlebotomus papatasi $x Biological control
- Phlebotomus papatasi $x Reproduction $x Regulation
- Leishmaniasis, Cutaneous $x Prevention