Effect of combined antioxidant supplementation on oxidative stress in myocardium from rats

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Linh Trong Nguyen (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Allan H Goldfarb

Abstract: "The purpose of this study was to determine the effects of a combined antioxidant supplementation on myocardial protein carbonyls (PC) and malondialdehyde (MDA) in rats after aerobic downhill running. Sixty-six male Sprague-Dawley rats were randomly assigned to either a normal diet or antioxidant supplemented diet (2,000 mg vitamin C + 1,000 IU vitamin E/kg diet) for 2 weeks. Exercised rats (N=48) ran on a rodent treadmill for 90 min at a speed of 16 m/min and - 16 degrees downhill grade. Rats were killed either at rest, immediately, 2 hrs, or 48 hrs post exercise. Rested rats were killed at the same times as the exercised rats all between 0700-1000 hrs. Ventricular tissue was analyzed for two markers of oxidative stress, PC and MDA. Only exercised rats that completed the 90 min run were included in the PC and MDA data analysis. There was a significant time main effect for PC concentration (p= 0.02) but post-hoc analysis indicated no specific time differences. However, PC approached significance (p=0.064) for the 2 hr post exercise time. There were no significant treatment or time*treatment effects for PC. MDA results showed no significant time, treatment or time*treatment effect. These data indicate that downhill running with modest intensity does not significantly increase oxidative stress in hearts as indicated by PC and MDA but a larger sample size is needed to confirm these results."--Abstract from author supplied metadata.

Additional Information

Publication
Thesis
Language: English
Date: 2007
Keywords
effects, antioxidant supplementation, myocardial protein carbonyls (PC), malondialdehyde (MDA), rats, aerobic, running
Subjects
Oxidative stress--Research
Diet therapy
Antioxidants