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Parke A. Rublee

**Ph.D., North Carolina State University**Microbial food chains in aquatic ecosystems, harmful algal blooms

There are 36 included publications by Parke A. Rublee :

TitleDateViewsBrief Description
Bacterial production in an arctic pond 1975 188 Bacteria of natural waters have an important role in recycling nutrients. As we accumulate information on the biomass of bacteria, however, we realize that these large quantities of bacteria may also be an important source of particulate matter for a...
Change of microplankton community structure in response to fertilization of an arctic lake 1995 263 Microplankton in an oligotrophic arctic lake were assessed by direct counts for one summer prior to nutrient additions and three summers during which inorganic nitrogen and phosphorus were added to the lake at approximately ten times ambient loading ...
Changes in abundance, composition and controls within the plankton of a fertilised arctic lake 2002 319 1. An oligotrophic arctic lake was fertilised with inorganic nitrogen and phosphorus as (NH4)2 NO3 and H3PO4 for five summers. The loading rate was 1.7–2.5 mmol N m–2 day–1 and 0.136–0.20 mmol P m–2 day–1 which is two to three times the annual loadin...
Characterization of Pfiesteria Ichthyocidal Activity 2005 150 Drgon et al. (4) concluded that the “aquarium bioassay format is unsuitable to accurately assess the ichthyocidal activity of Pfiesteria spp.” and “ichthyocidal activity of Pfiesteria spp. is mostly due to direct interactions of the zoospores with fi...
Community structure and bottom-up regulation of heterotrophic microplankton in arctic LTER lakes 1992 390 Microplankton community structures and abundance was assessed in lakes at the Toolik Lake LTER site in northern Alaska during the summers of 1989 and 1990. The microplankton community included oligotrich ciliates, but rotifers and zooplankton nauplii...
Decomposition of Turtlegrass (Thalassia testudinum Konig) in flowing seawater tanks and litterbags: Compositional changes and comparison with natural particulate matter 1982 289 Thalassia testudinum Konig litter was incubated up to 3 yr in flowing sea-water tanks and litterbags in the field. Weight loss of litter was evident within 1 wk and 50% loss of ash free dry weight took < 35 days. Carbon and nitrogen were lost from li...
Demonstration of toxicity to fish and to mammalian cells by Pfiesteria species: Comparison of assay methods and strains 2005 156 Toxicity and its detection in the dinoflagellate fish predators Pfiesteria piscicida and Pfiesteria shumwayae depend on the strain and the use of reliable assays. Two assays, standardized fish bioassays (SFBs) with juvenile fish and fish microassays ...
Detection of the Dinozoans Pfiesteria piscicida and P. shumwayae: A Review of Detection Methods and Geographic Distribution 2005 107 Molecular methods, including conventional PCR, real-time PCR, denaturing gradient gel electrophoresis, fluorescent fragment detection PCR, and fluorescent in situ hybridization, have all been developed for use in identifying and studying the distribu...
Detection of the Dinozoans Pfiesteria piscicida and P. shumwayae: A Review of Detection Methods and Geographic Distribution 2005 249 Molecular methods, including conventional PCR, real-time PCR, denaturing gradient gel electrophoresis, fluorescent fragment detection PCR, and fluorescent in situ hybridization, have all been developed for use in identifying and studying the distribu...
Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR 2007 237 The heterotrophic dinoflagellate Pfiesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-specific rea...
Detection of Pfiesteria spp. by PCR in surface sediments collected from Chesapeake Bay tributaries (Maryland) 2006 200 In 1997 blooms of Pfiesteria piscicida occurred in association with fish kills and human health problems in tributaries of the Chesapeake Bay (Maryland) and the scientific and media response resulted in large economic losses in seafood sales and tour...
Developing SSU rDNA metagenomic profiles of aquatic microbial communities for environmental assessments. 2008 609 Five water samples from three sources, two municipal reservoirs in central North Carolina and Toolik Lake in Alaska, were processed to conduct a comparative survey of microbial small subunit rDNA sequences. Genomic DNA was extracted and amplified by ...
Development and applications of microbial ecogenomic indicators for monitoring water quality: Report of a workshop assessing the state of the science, research needs and future directions 2006 446 This article brings forth recommendations from a workshop sponsored by the U.S. Environmental Protection Agency?s Science to Achieve Results (STAR) and Environmental Monitoring and Assessment (EMAP) Programs and by the Council of State Governments, h...
Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates 2000 194 Pfiesteria complex species are heterotrophic and mixotrophic dinoflagellates that have been recognized as harmful algal bloom species associated with adverse fish and human health effects along the East Coast of North America, particularly in its lar...
Direct Counts of Bacteria in the Sediments of a North Carolina Salt Marsh 1978 499 The number of bacteria in sediments of a North Carolina salt marsh was determined by direct counts with epifluorescent illumination and acridine orange stain. Cell number decreased from 8.36-10.90 × 109 cells/cm3 of sediment at the surface to 2.19-2....
Distribution and activity of bacteria in the headwaters of the Rhode River estuary, Maryland, USA 1984 173 A transect along the axis of the headwaters of a tidal estuary was sampled for microbial, nutrient, and physical parameters. Chlorophyll a averaged 42 µg 1-1 and phytoplankton comprised an estimated 80% of the total microbial biomass as determined by...
e of molecular probes to assess geographic distribution of Pfiesteria species 2001 100 We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts w...
Efficient utilization of dissolved free amino acids by suspended marine bacteria 1983 277 Incorporation of a mixture of 14C-labeled amino acids by bacteria averaged 79% of assimilation (incorporation plus respiration) but varied with time and location in the salinity gradient of the Newport River estuary, North Carolina, over a 9-month pe...
Geographic distribution of Pfiesteria spp. (Pfiesteriaceae) in Tasman Bay and Canterbury, New Zealand 2006 261 The fish-killing heterotrophic dinoflagellate species Pfiesteria piscicida and P. shumwayae (also Pseudopfiesteria shumwayae) were present throughout New Zealand and were residents of Tasman Bay?s well-flushed estuaries and Canterbury?s brackish lake...
Heteroduplex mobility assay-guided sequence discovery: Elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools 2000 188 The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in field and laboratory settings, and with a novel clinical syndrome of impaired cognition and memory disturbance among humans after presu...
Human health and environmental impacts from Pfiesteria: A Science-based rebuttal to Griffith (1999) 1999 370 David Griffith began his article, ?Exaggerating Environmental Health Risk: The Case of the Toxic Dinoflagellate Pfiesteria? (Human Organization 58:119-127). with a quotation by Angell (1995) which notes that assuming a connection between an effect an...
Insidious effects of a toxic estuarine dinoflagellate on fish survival and human health 1995 1121 The estuarine dinoflagellate Pfiesteria piscicida gen. et sp. nov. produces exotoxin(s) that can be absorbed from water or fine aerosols. Culture filtrate (0.22 µm porosity filters, >250 toxic flagellated cells/ml) induces formation of open ulcerativ...
Lake characteristics influence recovery of microplankton in arctic LTER lakes following experimental fertilization 2001 177 Lakes N-1 and N-2 at the Arctic Long Term Ecological Research site at Toolik Lake, Alaska, U.S.A. were fertilized with nitrogen and phosphorus for 5 and 6 years, respectively. The response and recovery of the microplankton community (protozoans, roti...
Merging storm water management with stream rehabilitation: Greensboro’s Lake Daniel pilot project 1995 271 In spite of progress controlling discharges of industrial pollutants from discrete points, many urban drainage basins continue to suffer from heavy loads of sediment and pollutants in the form of storm water runoff from lawns, streets, driveways, par...
A method to determine in situ zooplankton grazing rates on natural particle assemblages 1981 522 In situ zooplankton grazing rates on natural particle assemblages were estimated by measuring zooplankton uptake of labelled autotrophic (with Na14CO3) and heterotrophic (with [methyl-3H]-thymidine) particulate matter in 1-h incubations in clear, Ple...
Microfaunal response to fertilization of an arctic tundra stream 1995 317 1. As part of a whole-system study, the response of the heterotrophic microfaunal community colonizing artificial substrata (polyfoam units) to fertilization of an arctic tundra stream was followed for 6 weeks during the summer. 2. Dominant heterotr...
Nutrient flux in the Rhode River: Tidal transport of microorganisms in brackish marshes 1983 439 Concentrations of bacteria, chlorophyll a, and several dissolved organic compounds were determined during 11 tidal cycles throughout the year in a high and a low elevation marsh of a brackish tidal estuary. Mean bacterial concentrations were slightly...
Pfiesteria piscicida and Pfiesteria shumwayae in coastal waters of Long Island, New York, USA 2006 358 Water and sediment samples were collected during summer and early fall 1999–2004 from coastal waters of New York State, USA, to test for the presence of Pfiesteria piscicida and Pfiesteria shumwayae. Physical and chemical conditions were characterize...
Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand 2002 430 Pfiesteria shumwayae Steidinger et Burkholder is now known to be present in New Zealand and occurs in estuaries around the country. The presence of Pfiesteria was initially determined by a polymerase chain reaction (PCR)-based detection assay, using ...
Potential invasion of microorganisms and pathogens via ‘interior hull fouling’: biofilms inside ballast water tanks 2005 329 Surfaces submerged in an aquatic milieu are covered to some degree with biofilms – organic matrices that can contain bacteria, microalgae, and protozoans, sometimes including disease-causing forms. One unquantified risk of aquatic biological invasion...
Rotifers in arctic North America with particular reference to their role in microplankton community structure and response to ecosystem perturbations in Alaskan Arctic LTER lakes 1998 326 Growing interest in the development of mineral and recreational resources, along with the recognition that arctic ecosystems may be among those most affected by global change, has stimulated the study of arctic systems in recent decades. These have i...
Seasonal distribution of bacteria in salt marsh sediments of North Carolina 1982 806 The number and size of bacteria at four depths (0-1, 5-6, 10-11, and 20-21 cm) in a North Carolina salt marsh were minotored by direct counts for 13 months. The number of bacteria reached a maximum of about 1•4 × 1010 cells cm-3 at the sediment surfa...
Size of Suspended Bacterial Cells and Association of Heterotrophic Activity with Size Fractions of Particles in Estuarine and Coastal Waters 1984 283 The size of bacteria and the size distribution of heterotrophic activity were examined in estuarine, neritic, and coastal waters. The data indicated the small size of suspended marine bacteria and the predominance of free-living cells in numerical ab...
Taxonomy of Pfiesteria (Dinophyceae) 2006 580 The dinoflagellate species originally described as Pfiesteria shumwayae Glasgow et Burkholder, recently transferred to a new genus, Pseudopfiesteria Litaker et al., is reclassified into the redefined genus Pfiesteria Steidinger et Burkholder, as Pfie...
The transport of bacteria in sediments of a temperate marsh 1983 225 The number of bacteria in sediments, interstitial water and overlying tidal water of an oligohaline marsh system are about 109, 106, and 106 cells cm-3, respectively. Average cell size in the overlying water (about 0•06 µm3), is much smaller than tha...
Use of fluorescently labelled algae (FLA) to estimate microzooplankton grazing 1989 345 The fluorescent dye 5-(4,6-dichlorotriazin-2-yla)m inofluorescein (DTAF) was used to label a small alga (Nannochloris sp.), following the protocol of Sherr et al. (1987). A rotifer (Brachionus sp.) and a small ciliate (Euplotes sp.) ingested the fluo...