|Quantitative Measurements of Carcinogen-DNA Adduct Using MALDI Time-Of-Flight Mass Spectrometry
||Many carcinogens and their electrophilic metabolites react readily with DNA, and form different types of DNA adducts. Clinically, DNA adducts have been linked to cancer diseases. Also, different DNA adducts have been used as biomarkers to monitor the...
|Detection and sequencing of microRNA using MALDI time-of-flight mass spectrometry
||The discovery of microRNAs (miRNAs) and their abilities to regulate in vivo protein synthesis have led to growing interests in miRNA research. Due to their functions on regulating cellular processes that are related to diseases such as cancer, miRNAs...
|Mass spectrometric methods and bioinformatics tools for accurate identification of MicroRNA biomarkers
||MicroRNA (miRNA) are a class of endogenous non-protein-coding RNA of ~19-25 nucleotides long that post-transcriptionally regulate protein expression by targeting messenger RNAs for cleavage or translational repression. MiRNAs have been implicated in ...
|Investigating the role of plasma proteins in the cellular uptake of metallic nanoparticles
||In recent years applications of nanotechnology have increased at an unprecedented rate. Nanotechnology, which is defined as the engineering of matter on an atomic and molecular scale, has proven beneficial in a number of scientific fields. In particu...
|Whole cell mass spectroscopy as a cytotoxicity screening method
||Whole cell mass spectroscopy (WCMS) is a method that has gain popularity in the last decade. WCMS saves time by omitting extensive sample preparation for the mass spectral analysis of microorganisms. WCMS has been mainly used for profiling and identi...
|Cytotoxicity Screening Matrix Assisted Laser Desorption Ionization Mass Spectrometery for nanoparticle biomarker detection
||Toxicology is a broad topic that aims to assess the risk of chemicals on living organisms. It has been acknowledged that everything is toxic to a living organism. What distinguishes a toxin from a remedy depends on the dose 1. Presently, there are to...
|Detection of Argonaute (Ago) protein associated MiRNA by combining anti-ago antibody recognition with real-time PCR
||MicroRNAs (miRNAs) are small molecules of noncoding RNA that range between ~19-22 nucleotides in length. In recent years, scientists have observed that these small RNA molecules exist in the extracellular environment within eukaryotic organisms. Furt...