| Title | Date | Views | Brief Description |
|---|
| Molecular information storage using CRISPR |
2023 |
47 |
DNA (deoxyribonucleic acids) can be used as a digital data storage medium. Using its nucleotide sequence as a basis for storing digital data, DNA has a significantly higher data density and longer lifespan compared to silicon-based digital data stora... |
| Increasing CRISPR-mediated digital data storage in DNA using engineered “Janus” protospacers |
2023 |
149 |
DNA molecules can be used to store digital data if the sequence of their nucleotides is assigned to different values of ‘0’ or ‘1’. Rather than having to synthesize new DNA molecules for data storage on demand, our laboratory and its collaborators ha... |
| M.S. selective enrichment of rare mutations as a new biotechnology to study DNA mismatch repair processes in bacteria |
2020 |
422 |
DNA mismatch repair (MMR) is the key process which ensures the incorporation of correct nucleotides during DNA replication by recognizing and removing of incorrectly paired nucleotides from DNA. DNA replication can introduce a mismatched nucleotide a... |
| Screening highly-specific hairpin guide RNAs for CRISPR-Cas gene editing |
2021 |
272 |
This thesis outlines the development of a screening method to improve the specificity of the CRISPR effector, Cas9, in gene editing applications. We accomplish this by identifying modifications to the RNA-cofactor of Cas9, its guide RNA (gRNA), that ... |
| Engineering high-throughput approaches for CRISPR gene therapy and synthetic biology |
2024 |
43 |
The field of synthetic biology has witnessed rapid advancements in recent years, driven by the integration of high-throughput engineering approaches. This dissertation delves into the design, development, and implementation of high-throughput tools t... |