Use of an Improved Method for Analysis of Urinary Aflatoxin M 1 in a Survey of Mainland China and Taiwan

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Martin Root Ph.D, Associate Professor (Creator)
Appalachian State University (ASU )
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Abstract: An improved monoclonal antibody immunoaffinity chromatography/high-pressure liquid chromatography/ftuorescence detection method was developed to measure aftatoxin (AF) exposure by quantifying AFM 1 inhuman and rat urine samples.Analysis of different amounts of various AF metabolites showed that the immunoaffinity resin was highlyselective for aftatoxin 81 (AFB1), AFB2, and AFM 1• Recovery of added AFs increased with the amount of immunoaffinity resin and was virtually complete within the range of 0.01-10 ng of AFM 1 by using 7 ml of resin. The detection limit of this method is 0.5 pglml urine. Rats dosed with tritiated AFB 1 excreted in their urine tritiated AFM., among other AF metabolites, as indicated by chemical derivative confirmation and cochromatography with authentic AFM 1 and agreement of radioactivity and ftuorescence quantitation. Alinear dose-response relationship was found over the range of 0.05-50 p.glkg of body weight/day. Two humans dosed with 1.0 µ.g of pure AFB 1 excreted 6-7% of the dose asurinary AFM 1 over 5-7 days. Pooled urine samples from 30 men from each of 69 rural counties in mainland China and 16 survey areas in Taiwan, with two villages per county or area, were analyzed with this improved method (170 villages total). The correlationcoefficient of urinary excretion of AFM 1 compared between villages within all 85 survey areas was 0.50 (P <0.001). Sixty-five % of the samples contained detectable concentrations of AFM 1 with an average excretion of 3.1 ng/12 h. Assuming an excretion rate of 2-6%, this AFM 1 excretion corresponds to a very low average daily AF consumption of 0.1-0.3 p.g/day (possible range, 0-11 p.g/day). Patterns of urinary excretion of AFM 1 were similar in mainland China and Taiwan

Additional Information

Zhiqiang Cheng, Martin Root, Wenham Pan, Junshi Chen, and T. Colin Campbell(1997)Use of an Improved Method for Analysis of Urinary Aflatoxin M 1 in a Survey of Mainland China and Taiwan. Cancer Epidemiol Biomarkers Prev (volume#6 pg. 523-529)
Language: English
Date: 1997
, urinary, aflatoxin, china, taiwan,

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