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Impact of trans-10, cis-12 conjugated linoleic acid on interleukin (IL)-6 and IL-8 and adipogenic genes in cultures of human adipose tissue explants

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
James Nathan Provo (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Michael McIntosh

Abstract: "Obesity has reached epidemic proportions in the United States, currently ranking second among causes of preventable deaths. Our research group has shown in cultures of differentiated human adipocytes that 30 uM trans-10, cis-12 Conjugated Linoleic Acid (CLA) increases IL-6 and IL-8 secretion into the media and lowers mRNA levels of peroxisome proliferator-activated receptor gamma (PPAR). Human adipose tissue explants were incubated with either vehicle or 30 uM trans-10, cis-12 CLA for 8, 24, or 72 h. Trans-10, cis-12 CLA treatment increased IL-6 and IL-8 secretion into the media (p < 0.05). Trans-10, cis-12 CLA treatment also increased the mRNA levels of IL-6 and IL-8 (p < 0.05) while having no significant effect on PPARg or PPARg targets. These data demonstrate for the first time that CLA induces IL-6 and IL-8 gene expression and protein secretion in cultures of human subcutaneous adipose tissue explants"-Abstract from author supplied metadata.

Additional Information

Publication
Thesis
Language: English
Date: 2005
Keywords
Obesity, United States, preventable deaths, differentiated human adipocytes, Conjugated Linoleic Acid (CLA), Human adipose tissue explants, gene expression, protein secretion
Subjects
Obesity--Molecular aspects
Obesity--Genetic aspects
Linoleic acid