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Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Thomas Johnston (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Nicholas Oberlies

Abstract: Part I: Adsorbent resins are used in industrial fermentations to help boost the production of antibiotics. The resins used here were utilized to help speed the drug discovery process by eliminating the time-consuming step of freeze-drying the fermentation extract. This led to a shortened time span from receiving the fermentation to identifying the secondary metabolites via HPLC and LC/MS. Part II: Three new xanthones, 1-hydroxy-5-hydroxymethyl-6-methoxy-7-methyl-xanthone (1), 1-hydroxy-6-methoxy-7-methyl-xanthone (2), and 1-hydroxy-5,7-dimethyl-6-methoxy-xanthone (3), were isolated from an unknown fungus, MSX 68425, from the Mycosynthetix library of filamentous fungi. The compounds were discovered using bioactivity-directed fractionation, in pursuit of anticancer lead compounds. The structures of these compounds were determined by spectroscopic and spectrometric techniques, in conjunction with comparisons to the literature.

Additional Information

Publication
Thesis
Language: English
Date: 2012
Keywords
Actinomycin, Adsorbent resin, Cytotoxic, Filamentous fungi, Structure elucidation, Xanthone
Subjects
Drug development
Antineoplastic antibiotics $x Development
Filamentous fungi $x Biotechnology