Effects of suboptimal temperature on cell division in human lymphocytes in vitro
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Mary Frances Ballance (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Laura Anderton
Abstract: Studies were conducted on human lymphocytes in vitro aimed at ultimately defining the mechanism by which exposure to suboptimal temperature acts on cell division. Even though various effects of "cold" have been observed by several investigators, the mode of action of exposure to suboptimal temperature or the process of cell division is only vaguely understood. Studies conducted with "cold" adapted mammalian cell lines of L (mouse subcutaneous fibroblasts), Detroit-6 (human bone marrow) and Hela (human epidermoid carcinoma) lines have revealed that exposure to H°C influences certain activities in the dividing cultures more than others. These cell lines were isolated from the survivors of wild cell populations exposed repeatedly to 4°C for increasing time periods, and have been maintained from two to four vears. (Holeckova, et al, 1965) The three cold-adapted cell lines showed increased numbers of polyploid cells as compared to controls maintained at 37°C, which persisted for months after the last exposure. Immediately after U°C exposure, increased endoreduplication (re-duplication occuring during interphase, producing diplochromosomes which may be observed as the cell enters mitosis) was evident. The cold-adapted cell lines showed increased synthetic efficiency and increased growth rate which was not seen in other polyploid lines investigated. (Cerny, et al, 1965; Holeckova et al, 1967)
Effects of suboptimal temperature on cell division in human lymphocytes in vitro
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Created on 1/1/1968
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Additional Information
- Publication
- Honors Project
- Language: English
- Date: 1968