A chimeric human-cat fusion protein blocks cat-induced allergy

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Christopher Kepley, Associate Professor (Creator)
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/

Abstract: Animal allergens are an important cause of asthma and allergic rhinitis. We designed and tested a chimeric human-cat fusion protein composed of a truncated human IgG Fcg1 and the major cat allergen Fel d1, as a proof of concept for a new approach to allergy immunotherapy. This Fcg-Fel d1 protein induced dose-dependent inhibition of Fel d1-driven IgE-mediated histamine release from cat-allergic donors' basophils and sensitized human cord blood-derived mast cells. Such inhibition was associated with altered Syk and ERK signaling. The Fcg-Fel d1 protein also blocked in vivo reactivity in FcrRIa transgenic mice passively sensitized with human IgE antibody to cat and in Balb/c mice actively sensitized against Fel d1. The Fcg-Fel d1 protein alone did not induce mediator release. Chimeric human Fcg-allergen fusion proteins may provide a new therapeutic platform for the immune-based therapy of allergic disease.

Additional Information

Nature Medicine, 2005; 11(4):446-9.
Language: English
Date: 2005
allergen immunotherapy, animal allergens, chimeric fusion protein, mast cells, basophils

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