Differentiation of functional human mast cells from adipose derived stem cells and their application in allergo-oncology and the treatment of breast cancer
- UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
- Jesse David Plotkin (Creator)
- Institution
- The University of North Carolina at Greensboro (UNCG )
- Web Site: http://library.uncg.edu/
- Advisor
- Christopher Kepley
Abstract: Mast Cells are important yet poorly understood components of the immune system. Study of mast cells has been hampered by difficulty in obtaining primary cells, the lack of functional cell lines and the high degree of dissimilarity between human and murine mast cells. Adipose derived stem cells are abundant cells in the human body that are easily and relatively non-invasively obtained. Using media containing mediators secreted by skin derived mast cells, adipose derived stem cells were differentiated into functional mast cells. Mast cells differentiated from adipose derived stem cells were tested for their ability to respond to and kill cancer cells using an IgE-mediated targeting method. Mast cell induced cytotoxicity was observed as well as interactions between mast cells and cancer cells. Adipose derived mast cells may represent a platform for allergo-oncology.
Differentiation of functional human mast cells from adipose derived stem cells and their application in allergo-oncology and the treatment of breast cancer
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Created on 8/1/2018
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Additional Information
- Publication
- Dissertation
- Language: English
- Date: 2018
- Keywords
- Adipose Derived Stem Cell, Allergo-oncology, Breast Cancer, Differentiation, IgE, Mast Cell
- Subjects
- Mast cells
- Stem cells
- Adipose tissues
- Breast $x Cancer $x Treatment
- Breast $x Cancer $x Immunological aspects