Characterization Of The Norspermidine-Preferential Abc-Type Transporter Potabcd1 In V. Cholerae Biofilm Formation

ASU Author/Contributor (non-ASU co-authors, if there are any, appear on document)
Elizabeth Anne Villa (Creator)
Institution
Appalachian State University (ASU )
Web Site: https://library.appstate.edu/
Advisor
Ece Karatan

Abstract: This study further characterizes the Vibrio cholerae norspermidine/spermidine ABC-type transporter PotABCD1. The role of this transporter in biofilm formation is relevant to two diverse phases of the V. cholerae life cycle: survival in the aquatic environment, and colonization within the human host. Biofilms provide a colonization advantage in the mouse model, and may provide protection from acidic conditions encountered in the host prior to reaching the small intestine. This work identified PotA as an essential component of the norspermidine/spermidine ABC-type transporter PotABCD1 in V. cholerae, and determined that this transporter is strongly norspermidine-preferential. In this study I have shown that the potABCD2D1 genes are cotranscribed in an operon. I have also shown that mutations in PotA, PotB, PotC or PotD1 results in an increase in biofilm formation compared to wild type, indicating that spermidine uptake from tryptone media has negative effects on wild-type biofilm formation. Finally, I have shown that biofilm forming ability may provide a colonization advantage in the zebrafish intestine. This work further characterizes the novel norspermidine and spermidine transporter PotABCD1 in V. cholerae polyamine uptake, and provides insight into the role of this system in physiological aspects including biofilm formation and association with aquatic organisms.

Additional Information

Publication
Thesis
Villa, E. (2016). Characterization Of The Norspermidine-Preferential Abc-Type Transporter Potabcd1 In V. Cholerae Biofilm Formation. Unpublished Master’s Thesis. Appalachian State University, Boone, NC.
Language: English
Date: 2016
Keywords
Vibrio cholerae, Polyamine, Biofilm, ABC transporter, Norspermidine

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