Understanding genetic regulation of UV-B responses in Arabidopsis thaliana
- UNCW Author/Contributor (non-UNCW co-authors, if there are any, appear on document)
- Stephanie J. Smith (Creator)
- Institution
- The University of North Carolina Wilmington (UNCW )
- Web Site: http://library.uncw.edu/
- Advisor
- Ann Stapleton
Abstract: Light plays a major signaling role in plant growth and development, which
directly involves plant photoreceptors. UV-B radiation (280-320nm) is also an integral
component of sunlight that may cause damage to macromolecules or activate adaptive
responses. Using genes known to be UV-inducible from previous microarray
experiments, I measured gene expression after UV-B exposure using real-time PCR in
wild-type Arabidopsis seedlings, and then compared wild-type gene expression to
expression in a putative UV-B photoreceptor mutant. I also monitored the effect of UV-B
on growth, which was measured by leaf rosette diameter. In two experimental settings,
there were significant differences between treatments (Mylar and cellulose diacetate),
genotypes (mutant and wild-type), and their interaction. Based on these results, I
conclude that the candidate gene fits the phenotype of a UV-B photoreceptor and confers
increased growth in the greenhouse when the photoreceptor is present in plants exposed
to UV-B radiation.
Understanding genetic regulation of UV-B responses in Arabidopsis thaliana
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Created on 1/1/2009
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Additional Information
- Publication
- Thesis
- A Thesis Submitted to the University of North Carolina at Wilmington in Partial Fulfillment of the Requirement for the Degree of Masters of Science
- Language: English
- Date: 2009
- Keywords
- Arabidopsis thaliana--Effect of light on, Arabidopsis thaliana--Effect of ultraviolet radiation on, Arabidopsis thaliana--Genetics
- Subjects
- Arabidopsis thaliana -- Genetics
- Arabidopsis thaliana -- Effect of ultraviolet radiation on
- Arabidopsis thaliana -- Effect of light on