Whole cell mass spectroscopy as a cytotoxicity screening method

UNCG Author/Contributor (non-UNCG co-authors, if there are any, appear on document)
Reynaldo T. Diaz (Creator)
Institution
The University of North Carolina at Greensboro (UNCG )
Web Site: http://library.uncg.edu/
Advisor
Norman Chiu

Abstract: Whole cell mass spectroscopy (WCMS) is a method that has gain popularity in the last decade. WCMS saves time by omitting extensive sample preparation for the mass spectral analysis of microorganisms. WCMS has been mainly used for profiling and identifying fungi, bacteria and mammalian cells. We utilized WCMS as an assay for differentiating in vitro liver cell toxicity. In this study we focused on HepG2 cells, a liver carcinoma cell line, for WCMS profiling of untreated cells and dosed cells with toxins. We have optimized the sampling conditions for two different mass spectrometers and to obtained accurate and sensitive profiling of the whole liver cells. To verify that we were inducing a toxic response at the time of WCMS we screened our cells with the traditional cytotoxicity assays Lactate Dehydrogenase (LDH) or (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) MTT. This research lays the groundwork for a technique with great applications in pharmacology, toxicology, and medicinal chemistry.

Additional Information

Publication
Thesis
Language: English
Date: 2013
Keywords
HepG2, MALDI, MS, MTT
Subjects
Electron microscopy
Chemistry, Analytic

Email this document to