Oral microbe-host interactions: influence of ß-glucans on gene expression of inflammatory cytokines and metabolome profile

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Ramiro Mendonça Murata (Creator)

Luciano José Pereira (Creator)

Viviam de Oliveira Silva (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Background: The aim of this study was to evaluate the effects of ß-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. ß-glucan (10 µg/mL or 20 µg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant.\r\nResults: The relative expression of interleukin (IL)-1-a and IL-18 genes in HGF-1 decreased with 10 µg/mL or 20 µg/mL of ß-glucan, where as the expression of PTGS-2 decreased only with 10 µg/mL. The expression of IL-1-a increased with 20 µg/mL and that of IL-18 increased with 10 µg/mL in OBA-9\; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of ß-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 µg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 µg/mL of ß-glucan.\r\nConclusions: Treatment with ß-glucans positively modulated the immune response and production of metabolites.

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Language: English

Date: 2023

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