EFFECTS OF GERANIOL, LINALOOL, AND CITRONELLAL ON VIRULENCE FACTORS OF CANDIDA SPP.

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Nicole Cavaliere (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Purpose: To assess cell viability and antifungal effects of Geraniol, Linalool, and Citronellal in Fibroblasts and Candida spp. \n\nMethods: Three essential oils were assessed to find the Antifungal and cell viability properties. Human Gingival Fibroblast cells (1 x 105) were seeded in 24 well plates with DMEM medium + 10% FBS and incubated at 37°C in 5% CO2 for 24 hours. A Reductor of resazurin to resorufin was used to assess the cell viability. Candida spp. (C. albicans MYA-2876, C. glabrata CBS-138, C. tropicalis MYA-750, C. dubliniensis MYA-646) were evaluated when treated with 5 mg/mL diluting 10 folds to find the MIC/MFC of Geraniol, Linalool, and Citronellal. \n\nResults: The cell viability has been established and confirms that Geraniol’s IC50 = 223.5 µM (log[IC50] = 2.349 µM), Linalool’s IC50 = 46.41 µM (log[IC50] = 1.667 µM), and Citronellal’s IC50 = 93.31 µM (log[IC50] = 1.970 µM). MIC/MFC for Geraniol: C. albicans (792 µM/>500µM) C. tropicalis (4484µM/>50mM) C. glabrata (767µM/>50µM) C. dubliniensis (77.83µm/50µM) for Linalool: C. albicans (5549µM/>500µM) C. tropicalis (1134µM/>50mM) C. glabrata (159µM/>50µM) C. dubliniensis (7516µM/>500µM) for Citronellal: C. albicans (>50mM/>50mM) C. tropicalis (>50mM/>50mM) C. glabrata (>50mM/>50mM) C. dubliniensis (>50mM/50mM)\n\nConclusion: Geraniol and Linalool both showed antifungal properties at sub cytotoxic doses whereas Citronellal did not. This was unexpected as Geraniol, Linalool, and Citronellal have similar molecular makeups.

Additional Information

Publication

Thesis

Language: English

Date: 2023

Subjects

Candida\nGeraniol\nLinalool\nCitronellal

Email this document to