Genome-wide identification and characterization of HSP gene superfamily in whitefly (Bemisia tabaci) and expression profiling analysis under temperature stress

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Xin-Ru,Wang,Chao,Ban,Fei-Xue,Zhu,Dan-Tong,Liu,Shu- Wang (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Heat shock proteins (HSP) are essential molecular chaperones that play important roles in the stress stimulation of insects. Bemisia tabaci, a phloem feeder andinvasive species, can cause extensive crop damage through direct feeding and transmissionof plant viruses. Here we employed comprehensive genomics approaches to identity HSPsuperfamily members in the Middle East Asia Minor 1 whitefly genome. In total, we identified 26 Hsp genes, including three Hsp90, 17 Hsp70, one Hsp60 and five sHSP (smallheat shock protein) genes. The HSP gene superfamily of whitefly is expanded comparedwith the other five insects surveyed here. The gene structures among the same familiesare relatively conserved. Meanwhile, the motif compositions and secondary structures ofBtHsp proteins were predicted. In addition, quantitative polymerase chain reaction analysis showed that the expression patterns of BtHsp gene superfamily were diverse acrossdifferent tissues of whiteflies. Most Hsp genes were induced or repressed by thermal stress(40°C) and cold treatment (4°C) in whitefly. Silencing the expression of BtHsp70-6 significantly decreased the survival rate of whitefly under 45°C. All the results showed theHsps conferred thermo-tolerance or cold-tolerance to whiteflies that protect them frombeing affected by detrimental temperature conditions. Our observations highlighted themolecular evolutionary properties and the response mechanism to temperature assaults ofHsp genes in whitefly.

Additional Information

Publication

Other

Language: English

Date: 2019

Keywords

cold stress; comparative genomics; HSP; phylogenetic; thermal stress; whitefly

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