Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme -- phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Agnieszka,Grenda,Marcin,Mamczur,Piotr,Wisniewski,J A.,Wisniewski,Jacek R.,Rakus,Dariusz Gizak (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Phosphoglycerate mutase (PGAM), a conserved, glycolytic enzyme has beenfound in nucleoli of cancer cells. Here, we present evidence that accumulation ofPGAM in the nucleolus is a universal phenomenon concerning not only neoplasticallytransformed but also non-malignant cells. Nucleolar localization of the enzyme isdependent on the presence of the PGAM2 (muscle) subunit and is regulated by insulin/IGF-1--PI3K signaling pathway as well as drugs influencing ribosomal biogenesis. Wedocument that PGAM interacts with several 40S and 60S ribosomal proteins and thatsilencing of PGAM2 expression results in disturbance of nucleolar structure, inhibitionof RNA synthesis and decrease of the mitotic index of squamous cell carcinoma cells.We conclude that presence of PGAM in the nucleolus is a prerequisite for synthesisand initial assembly of new pre-ribosome subunits.

Additional Information

Publication

Other

Language: English

Date: 2015

Keywords

squamous cell carcinoma, PGAM2, rRNA, ribosome assembly, multifunctional enzyme

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