NMR method for monitoring changes in the core of lipoprotein particles in metabolism and disease

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

David Cistola (Creator)

Michelle Robinson (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: A method is disclosed for measuring the properties of protein and lipoprotein elements in a sample. The method includes the of placing a small volume of a sample into a NMR instrument tuned to measure a particular nucleus , applying a series of radiofrequency pulses with intermittent delays in order to measure spin-spin and/or spin-lattice relaxation time constants from the time-domain decay of the signal , without the use of chemical shifts and without converting data into the frequency domain by Fourier transform or other means , at least partially suppressing the water signal prior to the beginning of a sequence used to record relaxation time constants in the time domain , optionally utilizing relaxation contrast agents or other chemical additives to perturb the solvent water or other elements of the sample , analyzing the exponentially decaying NMR signal in the time domain using multi-exponential analysis , and comparing differences in the relaxation time constants for lipoprotein- or protein-specific elements within a single human subject , or between subjects , to assess normal and abnormal metabolism reflective of increased disease risk or active disease.

Additional Information

Publication

Other

Language: English

Date: 2017

Keywords

diagnostic, untransformed data, protein

Subjects

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