Identification of Damaged DNA Adducts from Exposure to Benzo[a]pyrene in the TP53 Gene

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Lea Taylor (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Extracted text; Benzo[a]pyrene is a carcinogen associated with tobacco smoke that can damage DNA after it is metabolized into highly reactive forms. Identifying the resulting DNA adducts can give greater insight into the mutations that frequently occur in lung cancer. This research aimed to identify damaged DNA adducts from exposure to benzo[a]pyrene in the TP53 gene. This gene codes for the p53 protein, which is frequently mutated in cancers. The oligomeric DNA sequence was exposed to myoglobin, benzo[a]pyrene, and hydrogen peroxide. Myoglobin acted as an heme enzyme mimic, and provided similar chemistry to bio-relevant cytochrome P450 enzymes that are involved in the metabolism of substances like benzo[a]pyrene. LC-MS was used to identify DNA adducts.

Additional Information

Publication

Thesis

Taylor, Lea. (2015). Identification of Damaged DNA Adducts from Exposure to Benzo[a]pyrene in the TP53 Gene. Unpublished manuscript, Honors College, East Carolina University, Greenville, NC.

Language: English

Date: 2015

Keywords

Benzo[a]pyrene, DNA adducts

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