Th1 Adjuvant N-Acetyl-d-Glucosamine Polymer Up-Regulates Th1 Immunity but Down-Regulates Th2 Immunity against a Mycobacterial Protein (MPB-59) in Interleukin-10-Knockout and Wild-Type Mice

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Ikuro Honda (Creator)

J. Paul Justice (Creator)

Quentin N. Myrvik (Creator)

Reiko M. Nakamura (Creator)

Yoshimi Shibata (Creator)

Scott Michael R. Van (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Treatment of mice with heat-killed (HK) Mycobacterium bovis BCG or 1- to 10-μm chitin particles (nonantigenic N-acetyl-d-glucosamine polymers) is known to induce innate immune responses including gamma interferon (IFN-γ) production which plays a Th1 adjuvant role. However HK BCG further induces prostaglandin E2-releasing spleen macrophages (Mφ) (PGE2-Mφ) which potentially inhibit Th1 adjuvant activities. We found that chitin particles did not induce PGE2-Mφ formation. To further assess whether chitin has Th1 adjuvant effects interleukin-10 (IL-10)-knockout (KO) mice and their wild-type (WT C57BL/6) controls were immunized with a 30-kDa MPB-59 mycobacterial protein mixed with chitin. Immunization with MPB-59 alone induced Th2 responses characterized by increases in total serum immunoglobulin E (IgE) and specific serum IgG1 levels and spleen Th2 cells producing IL-4 IL-5 and IL-10. No IFN-γ-producing spleen Th1 cells specific serum IgG2a or delayed-type hypersentivity (DTH) footpad reactions were detected. On the other hand chitin–MPB-59 immunization significantly increased spleen Th1 responses DTH reaction and serum IgG2a levels along with decreases of Th2 responses. The magnitude of these Th1 adjuvant effects was greater in IL-10-KO mice than in WT mice. In contrast immunization with HK BCG–MPB-59 showed little or no Th1 adjuvant effect. These data indicate that chitin has a unique Th1 adjuvant effect on the development of Th1 immunity against a mycobacterial antigen. IL-10 down-regulates the adjuvant effect of chitin. Originally published Infection and Immunity Vol. 69 No. 10 Oct 2001

Additional Information

Publication

Other

Infection and Immunity. 69:10(October 2001) p. 6123-6130.

Language: English

Date: 2011

Keywords

mycobacterial protein, Th1 immunity, Th2 Immunity, glucosamine polymer

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