PI(4 5)P2 concentration at the APC side of the Immunological Synapse is Required for Effector T cell Function

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Sarah Boyle (Creator)

Michael Edidin (Creator)

David Robert Fooksman (Creator)

Saame Raza Shaikh (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Little is known about the signaling that occurs in an antigen presenting cell (APC) during contact with a T cell. Here we report the concentration of the signaling lipid PI(4 5)P2 at the APC side of the immunological synapse. In both human and mouse cells a PI(4 5)P2-specific fluorescent reporter PH-GFP detected an antigen-dependent enrichment of PI(4 5)P2 at the synapse between antigen- specific T cells and APC. When PIP(4 5)P2 was sequestered by a high concentration of PH-GFP reporter cells were less susceptible to CTL-mediated lysis than control cells. These findings suggest a new regulatory target for modulating immune function that may be exploited for immune escape by pathogens and tumors. Originally published Journal of Immunology Vol. 182 No. 9 May 2009

Additional Information

Publication

Other

Journal of Immunology. 182:9(May 2009) p. 5179-5182.

Language: English

Date: 2011

Keywords

cytotoxicity, T cells cytotoxic, MHC, Antigen Presentation

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