Identification classification and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Jason Bond (Creator)

Peter Thomas (Creator)

Yong Zhu (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Recently we discovered a previously uncharacterized gene with the characteristics of a membrane progestin receptor (mPR) in a fish model spotted seatrout. Here we report the identification cloning and characteristics of other members of this hitherto unknown family of putative mPRs from several vertebrate species including human mouse pig Xenopus zebrafish and Fugu with highly conserved nucleotide and deduced amino acid sequences and similar structures to the spotted seatrout mPR. The 13 vertebrate genes identified seem to belong to an unknown gene family. Phylogenetic analysis indicates these cDNAs comprise three distinct groups (named alpha beta and gamma) within this gene family. Structural analyses of the translated cDNAs suggest they encode membrane proteins with seven transmembrane domains. The transcript sizes of the human alpha beta and gamma putative mPR mRNAs varied from 2.8 to 5.8 kb and showed distinct distributions in reproductive neural kidney and intestinal tissues respectively. Recombinant human alpha gamma and mouse beta proteins produced in an Escherichia coli expression system demonstrated high affinity (Kd = 20 - 30 nM) saturable binding for progesterone. Further analysis of binding to the gamma - subtype revealed binding was specific for progestins and was displaceable with rapid rates of association and dissociation (t1/2 = 2 - 8 min). These results suggest this is a new family of steroid receptors unrelated to nuclear steroid receptors but instead having characteristics of G protein-coupled receptors. Originally published Proceedings of the National Academy of Sciences Vol. 100 No. 5 2237-2242

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Publication

Other

Proceedings of the National Academy of Sciences. 100:5(March 2003) p. 2237-2242.

Language: English

Date: 2011

Keywords

membrane progestin receptor, steroid receptors, cDNA

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