Identification and mapping of two divergent unlinked major histocompatibility complex class II B genes in Xiphophorus fishes.

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Ulla B. Godwin (Creator)

Steven Kazianis (Creator)

Thomas J. McConnell (Creator)

Donald C. Morizot (Creator)

Rodney S. Nairn (Creator)

Stephen F. Norton (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: We have isolated two major histocompatibility complex (MHC) class II B genes from the inbred fish train Xiphophorus maculatus Jp 163 A. We mapped one of these genes designated here as DXB to linkage roup III linked to a malic enzyme locus also syntenic with human and mouse MHC. Comparison of enomic and cDNA clones shows the gene consists of six exons and five introns. The encoded b1 domain as three amino acids deleted and a cytoplasmic tail nine amino acids longer than in other teleost class I b chains more similar to HLA-DRB clawed frog Xela-F3 and nurse shark Gici-B. Key residues for isulfide bonds glycosylation and interaction with a chains are conserved. These same features are also resent in a swordtail (Xiphophorus helleri) genomic DXB PCR clone. A second type of class II B clone was mplified by PCR from X. maculatus and found to be orthologous to class II genes identified in other ishes. This DAB-like gene is 63% identical to the X. maculatus DXB sequence in the conserved b2-encoding xon and was mapped to new unassigned linkage group LG U24. The DXB gene then represents an nlinked duplicated locus not previously identified in teleosts. Originally published Genetics Vol. 149 No. 4 August 1998

Additional Information

Publication

Other

Genetics. 149:4(August 1998) p. 1921-1934.

Language: English

Date: 2011

Keywords

xiphophorus fishes, histocompatibility complex, gene mapping

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