Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Larry A. Gallagher (Creator)

Marina S. Kuznetsova (Creator)

Colin Manoil (Creator)

Susan L. McKnight (Creator)

Everett C. Pesci (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in the primary phenazine biosynthetic pathway and those with more pleiotropic defects. The largest set of pleiotropic mutations blocked the production of the extracellular Pseudomonas quinolone signal (PQS) a molecule required for the synthesis of secondary metabolites and extracellular enzymes. Most of these pqs mutations affected genes which appear to encode PQS biosynthetic functions although a transcriptional regulator and an apparent response effector were also represented. Two of the genes required for PQS synthesis (phnA and phnB) had previously been assumed to encode phenazine biosynthetic functions. The transcription of one of the genes required for PQS synthesis (PA2587/pqsH) was regulated by the LasI/R quorum-sensing system thereby linking quorum sensing and PQS regulation. Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network including one regulator (np20) previously shown to be required for virulence in neutropenic mice. Originally published Journal of Bacteriology Vol. 184 No. 23 Dec 2002

Additional Information

Publication

Other

Journal of Bacteriology. 184:23(December 2002) p. 6472-6480.

Language: English

Date: 2011

Keywords

Pseudomonas aeruginosa, quinolone signaling, phenazine biosynthetic pathway

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