Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins

ECU Author/Contributor (non-ECU co-authors, if there are any, appear on document)

Shaw M. Akula (Creator)

Ossie F. Dyson (Creator)

Patrick W. Ford (Creator)

Khalief E. Hamden (Creator)

Telisha L. Oxendine (Creator)

Institution

East Carolina University (ECU )

Web Site: http://www.ecu.edu/lib/

Abstract: Kaposi’s sarcoma-associated herpesvirus (KSHV) has two modes replication: latent and lytic replication. Reactivation from latency is dictated in part by the cell cycle. Herein we have attempted to delineate the importance of cell cycle in KSHV pathogenesis by exploring the expression pattern of cell surface receptors during different phases of the cell cycle. αV integrin expression is augmented uring S phase in fibroblasts epithelial and KSHV infected cells. Using a Matrigel system we pioneer the concept that KSHV infected primary effusion lymphoma (PEL) cells can attach to extracellular matrix proteins. This attachment is mediated primarily via αV integrins or virally encoded gB and occurs preferentially in cells from S phase or cells from S phase actively supporting a lytic infection respectively. Such an ability of infected B cells to attach to endothelial cells may lso aid in the dissemination of infection. The keystone of this work is that for the first time we describe the ability of KSHV infected B cells to preferentially use cellular (αV) or viral (gB) receptors to specifically bind cells depending upon the stage of the cell cycle and infection. Originally published Cellular Microbiology Vol. 10 No. 7 July 2008

Additional Information

Publication

Other

Cellular Microbiology. 10:7(July 2008) p. 1546-1558.

Language: English

Date: 2011

Keywords

KSHV, HHV-8, cellular attachment, gB, alpha-V, Integrins, Matrigel, Reactivation

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